MiR-125b-5p/STAT3轴通过作用于ABC转运蛋白调控骨肉瘤细胞的耐药性。
MiR-125b-5p/STAT3 Axis Regulates Drug Resistance in Osteosarcoma Cells by Acting on ABC Transporters.
作者信息
Yang Yang, Chen Yueyuan, Liu Jiajia, Zhang Bo, Yang Linlin, Xue Jianhua, Zhang Zexu, Qin Lili, Bian Rongpeng
机构信息
Department of Trauma Center, Affiliated Hospital of Nantong University, Nantong City, Jiangsu Province 226001, China.
Department of Oncology, Second People's Hospital of Nantong, Nantong City, 226001 Jiangsu Province, China.
出版信息
Stem Cells Int. 2023 Apr 29;2023:9997676. doi: 10.1155/2023/9997676. eCollection 2023.
BACKGROUND
The poor prognosis of the highly malignant tumor osteosarcoma stems from its drug resistance and therefore exploring its resistance mechanisms will help us identify more effective treatment options. However, the effects of miR-125b-5p on drug resistance in osteosarcoma cells are still unclear.
METHODS
To study the effects of miR-125b-5p on drug resistance in osteosarcoma cells. Osteosarcoma-resistant miR-125b-5p was obtained from the databases GeneCards and g:Profiler. CCK8, western blot, and transwell were applied for the detection of the miR-125b-5p effects on proliferation, migration, invasion, apoptosis, and drug resistance in osteosarcoma. Bioinformatics is aimed at demonstrating the targeting factor miR-125b-5p, performing protein interaction enrichment analysis by Metascape, and finally validating by binding sites.
RESULTS
Upregulation of miR-125b-5p restrains proliferation, migration, and invasion of osteosarcoma and promotes apoptosis. In addition, miR-125b-5p can restore drug sensitivity in drug-resistant osteosarcoma. miR-125-5p restrains the signal transducer and inhibits the transcription 3 (STAT3) expression activator via targeting its 3'-UTR. STAT3 affects drug-resistant osteosarcoma to regulate the ABC transporter.
CONCLUSION
miR-125b-5p/STAT3 axis mediates the drug resistance of osteosarcoma by acting on ABC transporter.
背景
高恶性肿瘤骨肉瘤预后较差，源于其耐药性，因此探索其耐药机制将有助于我们确定更有效的治疗方案。然而，miR-125b-5p对骨肉瘤细胞耐药性的影响仍不清楚。
方法
为研究miR-125b-5p对骨肉瘤细胞耐药性的影响。从GeneCards和g:Profiler数据库中获取骨肉瘤耐药性相关的miR-125b-5p。应用CCK8、蛋白质印迹法和Transwell实验检测miR-125b-5p对骨肉瘤细胞增殖、迁移、侵袭、凋亡及耐药性的影响。生物信息学旨在展示靶向因子miR-125b-5p，通过Metascape进行蛋白质相互作用富集分析，最后通过结合位点进行验证。
结果
miR-125b-5p的上调抑制骨肉瘤的增殖、迁移和侵袭，并促进凋亡。此外，miR-125b-5p可恢复耐药性骨肉瘤的药物敏感性。miR-125-5p通过靶向信号转导和转录激活因子3（STAT3）的3'-UTR抑制其表达。STAT3影响耐药性骨肉瘤以调节ABC转运蛋白。
结论
miR-125b-5p/STAT3轴通过作用于ABC转运蛋白介导骨肉瘤的耐药性。
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