Edmonds P D, Ross P
Ultrasound Med Biol. 1986 Apr;12(4):297-305. doi: 10.1016/0301-5629(86)90339-x.
Cell viability, survival, and growth of C1300 mouse neuroblastoma cells were assayed by trypan blue dye exclusion, clonogenesis, and culturing, respectively, after exposure in suspension to therapeutic levels of ultrasound (1 MHz; continuous wave; spatial peak intensity 0.9, 1.7, and 2.6 W/cm2; 5 min at 37 degrees C). Acoustic emission from the cavitating cell suspensions was recorded as the rms value of the half-harmonic and noise components combined. Cell viability and survival appeared better correlated with acoustic emission than with spatial peak intensity, implying that acoustic emission may provide a more direct measure of insult to the cells in a cavitating field than the incident intensity. Biological assay results of growth were well correlated with spatial peak intensity but not with acoustic emission levels, which seems to imply that for this end point incident intensity is a more directly interacting parameter than cavitational activity, provided however that the latter is present. Further refinement of the technique for measuring acoustic emission from cell suspensions should permit separate measurements of the half-harmonic and noise components. When thus refined, it may provide the means to demonstrate cavitational action without resorting to additional experiments to suppress cavitation.
将C1300小鼠神经母细胞瘤细胞悬浮暴露于治疗水平的超声（1兆赫；连续波；空间峰值强度0.9、1.7和2.6瓦/平方厘米；37摄氏度下5分钟）后，分别通过台盼蓝染料排斥法、克隆形成法和培养法测定细胞活力、存活率和生长情况。记录空化细胞悬液的声发射，作为半谐波和噪声成分组合的均方根值。细胞活力和存活率与声发射的相关性似乎比与空间峰值强度的相关性更好，这意味着在空化场中，声发射可能比入射强度更能直接衡量对细胞的损伤。生长的生物学测定结果与空间峰值强度密切相关，但与声发射水平无关，这似乎意味着对于这个终点，入射强度比空化活动是一个更直接的相互作用参数，不过前提是空化活动存在。进一步完善测量细胞悬液声发射的技术，应能分别测量半谐波和噪声成分。如此完善后，它可能提供一种手段，无需借助额外的抑制空化实验就能证明空化作用。
