Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2023 Apr;174(6):806-809. doi: 10.1007/s10517-023-05794-2. Epub 2023 May 9.
We studied the neutrophils and monocytes obtained from 37 patients with various inflammatory diseases such as psoriasis, acute infectious process in the abdominal cavity (acute appendicitis/abscess of the abdominal cavity, and acute cholecystitis), acute pancreatitis, and post-COVID syndrome after mild COVID infection. The number and the morphological structure of neutrophil extracellular traps (NET) as well as the effect of IgG on NET were examined. NET were visualized and counted by fluorescence microscopy with fluorescent dye SYBR Green. All the studied types of inflammation were accompanied by spontaneous formation of NET. After application of IgG, the number of NET doubled, their size increased, and transformation of net-like traps into the cloud forms was observed. The clouds form structure of the network is not capable of capturing pathogens with subsequent retraction, the products of its enzymatic degradation can be the factors of secondary alteration. The study results demonstrate a previously unknown mechanism of infection resistance.
我们研究了来自 37 名患有各种炎症性疾病（如银屑病、腹腔急性感染过程（急性阑尾炎/腹腔脓肿、急性胆囊炎）、急性胰腺炎和轻度 COVID 感染后的新冠后综合征）患者的中性粒细胞和单核细胞。检查了中性粒细胞细胞外陷阱 (NET) 的数量和形态结构以及 IgG 对 NET 的影响。使用荧光染料 SYBR Green 通过荧光显微镜可视化和计数 NET。所有研究类型的炎症均伴有 NET 的自发形成。应用 IgG 后，NET 的数量增加了一倍，其大小增加，并观察到网状陷阱向云状的转化。云状结构的网络无法捕获随后缩回的病原体，其酶降解产物可能是继发改变的因素。研究结果表明了一种以前未知的抗感染机制。
