Hunan Provincial Key Laboratory of Pesticide Biology and Precise Use Technology, Hunan Agricultural Biotechnology Research Institute, Changsha, 410125, People's Republic of China.
College of Plant Protection, Hunan Agricultural University, Changsha, 410125, China.
Arch Microbiol. 2023 Apr 25;205(5):206. doi: 10.1007/s00203-023-03540-w.
Botrytis cinerea is a non-host-specific phytopathogenic fungus capable of infecting numerous cash crops. Here, we analyzed the functions of the Bcb1 gene in B. cinerea, which encodes a membrane protein belonging to the acyl-coenzyme A synthase family. Compared to the wild type, Bcb1-deletion mutants exhibited obvious morphological abnormalities, including slower vegetative growth and reduced melanin production. The absence of Bcb1 causes B. cinerea to form only small and incompletely developed infection cushions and fail to produce spores. The Bcb1 mutants displayed hypersensitivity to the membrane stressor SDS, the cell wall stressor Congo red, and the oxidative stressor HO and increased resistance to intracellular osmotic stress caused by KCl compared to the wild-type strain. However, there were no differences in tolerance to extracellular osmotic stress caused by NaCl. The deletion of Bcb1 also caused a reduction in pathogenicity. The qRT‒PCR results showed that the genes Bcpks12 and Bcpks13, which are related to melanin biosynthesis, and Bcpg2, BcBOT2, and cutA, which are related to virulence, were downregulated in ∆Bcb1. These data suggest that BCB1 is important for conidial morphogenesis, and pathogenesis in B. cinerea.
灰葡萄孢是一种非专性寄生的植物病原真菌，能够感染许多经济作物。在这里，我们分析了 Bcb1 基因在灰葡萄孢中的功能，该基因编码一种属于酰基辅酶 A 合成酶家族的膜蛋白。与野生型相比，Bcb1 缺失突变体表现出明显的形态异常，包括生长缓慢和黑色素生成减少。Bcb1 的缺失导致灰葡萄孢只能形成小而不完全发育的侵染垫，并且无法产生孢子。与野生型菌株相比，Bcb1 突变体对膜应激剂 SDS、细胞壁应激剂刚果红以及氧化应激剂 HO 表现出超敏反应，并且对 KCl 引起的细胞内渗透压应激的抗性增加，但对 NaCl 引起的细胞外渗透压应激的耐受性没有差异。Bcb1 的缺失也导致了致病性的降低。qRT-PCR 结果表明，与黑色素生物合成有关的基因 Bcpks12 和 Bcpks13 以及与毒性有关的基因 Bcpg2、BcBOT2 和 cutA 在 ∆Bcb1 中下调。这些数据表明 BCB1 对于灰葡萄孢分生孢子形态发生和致病性很重要。
