U.S. Department of Agriculture-Agricultural Research Service, Peanut and Small Grains Research Unit, Stillwater, OK 74075.
U.S. Department of Agriculture-Agricultural Research Service, Plant Science Research Unit, Raleigh, NC 27695.
Phytopathology. 2023 Oct;113(10):1979-1984. doi: 10.1094/PHYTO-01-23-0032-R. Epub 2023 Nov 10.
Powdery mildew is caused by the highly adaptive biotrophic fungus f. sp. infecting wheat worldwide. Novel powdery mildew resistance genes are urgently needed that can be used rapidly in wheat cultivar development with minimal disruption of trait advances elsewhere. PI 351817 is a German cultivar exhibiting a wide spectrum of resistance to f. sp. isolates collected from different wheat-growing regions of the United States. Evaluation of an F population and 237 F lines derived from OK1059060-2C14 × PI 351817 for responses to f. sp. isolate --- identified a single dominant gene, designated , for powdery mildew resistance in PI 351817. Using bulked segregant analysis (BSA) and simple sequence repeat (SSR) markers, was mapped in the terminal region of the long arm of chromosome 2A. Deep sequencing of the genotyping-by-sequencing libraries of the two parental lines identified a set of single-nucleotide polymorphism (SNP) markers in the 2AL candidate gene region. Those SNP markers was subsequently converted to Kompetitive allele-specific PCR (KASP) markers for genotyping the mapping population. Linkage analysis delimited to a 634-kb interval between Stars-KASP656 (771,207,512 bp) and Stars-KASP662 (771,841,609 bp) on 2AL, based on the Chinese Spring reference sequence IWGSC RefSeq v 2.1. Tests of allelism indicated that is located at the locus. shows resistance to all f. sp. isolates used in this study and can be used to enhance powdery mildew resistance in the United States. KASP markers flanking can be used to select in wheat breeding programs after further tests for polymorphism.
白粉病是由高度适应的生物营养真菌 f. sp. 引起的，它感染了全世界的小麦。迫切需要新的抗白粉病基因，可以在小麦品种开发中快速使用，而对其他性状的进展影响最小。PI 351817 是一种德国品种，对从美国不同小麦种植区采集的 f. sp. 分离物表现出广谱抗性。对 OK1059060-2C14×PI 351817 衍生的 F 群体和 237 条 F 系进行评价，以响应 f. sp. 分离物 --- 鉴定出一个单一的显性基因，命名为 ，用于 PI 351817 对白粉病的抗性。利用分离群体分组分析法（BSA）和简单重复序列（SSR）标记，将 定位在染色体 2A 长臂的末端区域。对两个亲本系的基因分型测序文库进行深度测序，在 2AL 候选基因区域鉴定出一组单核苷酸多态性（SNP）标记。随后，将这些 SNP 标记转化为 Kompetitive allele-specific PCR（KASP）标记，用于对作图群体进行基因分型。连锁分析将 限制在 2AL 上 Stars-KASP656（771,207,512 bp）和 Stars-KASP662（771,841,609 bp）之间的 634-kb 区间内，基于中国春参考序列 IWGSC RefSeq v 2.1。等位基因测试表明， 位于 位点。 对本研究中使用的所有 f. sp. 分离物均表现出抗性，可用于增强美国的白粉病抗性。侧翼的 KASP 标记可用于在进一步测试多态性后，在小麦育种计划中选择 。
