School of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.
Research Center of Biotechnology, Hebei University, Baoding, China.
Pest Manag Sci. 2023 Oct;79(10):3538-3547. doi: 10.1002/ps.7535. Epub 2023 May 29.
The Asian longhorned beetle (ALB), Anoplophora glabripennis, is a serious wood borer of hardwood trees. Populus deltoides 'Shalinyang' (PdS) is attractive to ALB adults for oviposition but highly resistant to their offspring. Investigation of the chemicals regulating ALB oviposition is scarce in previous studies until now. To determine which chemicals emitted by PdS were attractive and induced oviposition behavior by ALB on non-host poplar tree species, we first: collected and identified the bio-active volatiles produced by PdS using coupled gas chromatography-mass spectrometry (GC-MS) and coupled gas chromatography-electroantennographic detector (GC-EAD); then evaluated which chemicals were attractive in a Y-tube olfactometer bioassay; and finally screened key compounds affecting ALB oviposition using a 'chemical-stimulated oviposition on non-host tree' bioassay.
(E)-2-Hexenal, hexyl acetate, (Z)-3-hexenol acetate, 1-hexanol, (Z)-3-hexenol, β-caryophyllene, and salicylaldehyde emitted from PdS were attractive to ALB. When (E)-2-hexenal, 1-hexanol, (Z)-3-hexenol acetate, and (Z)-3-hexenol were applied to the non-host tree Populus tomentosa, oviposition by ALB females was significantly increased. Furthermore, the mean number of oviposition pits increased as the (Z)-3-hexenol concentrations increased. Further tests on synergy between pairs of chemicals showed that (Z)-3-hexenol stimulated production of the most oviposition pits, but that the percentage of effective oviposition pits (those containing an egg and larva and not empty) decreased.
(Z)-3-Hexenol is the main chemical component inducing ALB oviposition. These results increase understanding about the oviposition behavior of ALB and could help improve management strategies that regulate ALB behavior by planting mixed-species forests resistant to ALB. © 2023 Society of Chemical Industry.
亚洲长角天牛（ALB），Anoplophora glabripennis，是一种严重的硬木树蛀虫。杨树品种‘Shalinyang’（PdS）对 ALB 成虫产卵具有吸引力，但对其后代具有高度抗性。在以前的研究中，对调节 ALB 产卵的化学物质的研究很少。为了确定 PdS 释放的哪些化学物质对非宿主杨树品种的 ALB 产卵具有吸引力并诱导其产卵行为，我们首先：使用气相色谱-质谱联用仪（GC-MS）和气相色谱-触角电位仪（GC-EAD）联用收集和鉴定 PdS 产生的生物活性挥发物；然后在 Y 型嗅觉仪生物测定中评估哪些化学物质具有吸引力；最后使用“非宿主树上的化学刺激产卵”生物测定筛选影响 ALB 产卵的关键化合物。
PdS 释放的（E）-2-己烯醛、己酸乙酯、（Z）-3-己烯醇乙酸酯、1-己醇、（Z）-3-己烯醇、β-石竹烯和水杨醛对 ALB 具有吸引力。当（E）-2-己烯醛、1-己醇、（Z）-3-己烯醇乙酸酯和（Z）-3-己烯醇施用于非宿主树毛白杨时，ALB 雌虫的产卵量显著增加。此外，随着（Z）-3-己烯醇浓度的增加，产卵孔的平均数量也增加。进一步测试化学物质对协同作用的影响表明，（Z）-3-己烯醇刺激产卵孔数量最多，但有效产卵孔（含有卵和幼虫且不空）的百分比降低。
（Z）-3-己烯醇是诱导 ALB 产卵的主要化学物质成分。这些结果增加了对 ALB 产卵行为的理解，并有助于通过种植对 ALB 具有抗性的混种林来改善调节 ALB 行为的管理策略。© 2023 化学工业协会。
