del Val M, Carrascosa J L, Viñuela E
Virology. 1986 Jul 15;152(1):39-49. doi: 10.1016/0042-6822(86)90369-7.
Extracellular African swine fever (ASF) virus particles were specifically agglutinated by several lectins, suggesting the presence of surface glycosylated component(s) containing at least glucose, mannose, or both; galactose, N-acetylgalactosamine, or both; N-acetylneuraminic acid and N-acetylglucosamine, but not fucose. When virions were purified from infected Vero cells labeled with [14C]glucosamine, [14C]galactose and analyzed by polyacrylamide gel electrophoresis, no major structural glycoproteins were detected. However, several species of glycolipids were found when virions were extracted with organic solvents and analyzed by thin layer chromatography. These, plus two minor glycosylated structural components, of apparent mol wt 230K and 95K, could account for the agglutination of ASF virions with concanavalin A.
细胞外非洲猪瘟（ASF）病毒颗粒可被几种凝集素特异性凝集，这表明存在至少含有葡萄糖、甘露糖或两者的表面糖基化成分；半乳糖、N-乙酰半乳糖胺或两者；N-乙酰神经氨酸和N-乙酰葡糖胺，但不含有岩藻糖。当从用[14C]葡糖胺、[14C]半乳糖标记的感染Vero细胞中纯化病毒粒子并通过聚丙烯酰胺凝胶电泳分析时，未检测到主要的结构糖蛋白。然而，当用有机溶剂提取病毒粒子并通过薄层色谱分析时，发现了几种糖脂。这些糖脂，加上两种表观分子量分别为230K和95K的次要糖基化结构成分，可能解释了ASF病毒粒子与伴刀豆球蛋白A的凝集现象。
