National Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, Southern University of Science and Technology, 29 Bulan Road, Shenzhen, Guangdong, 518112, China.
Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, LA, 70112, USA.
Adv Sci (Weinh). 2023 Jul;10(20):e2301697. doi: 10.1002/advs.202301697. Epub 2023 May 10.
Numerous groups have employed the special properties of CRISPR/Cas systems to develop platforms that have broad potential applications for sensitive and specific detection of nucleic acid (NA) targets. However, few of these approaches have progressed to commercial or clinical applications. This review summarizes the properties of known CRISPR/Cas systems and their applications, challenges associated with the development of such assays, and opportunities to improve their performance or address unmet assay needs using nano-/micro-technology platforms. These include rapid and efficient sample preparation, integrated single-tube, amplification-free, quantifiable, multiplex, and non-NA assays. Finally, this review discusses the current outlook for such assays, including remaining barriers for clinical or point-of-care applications and their commercial development.
许多研究小组利用 CRISPR/Cas 系统的特殊性质开发了各种平台，这些平台在灵敏和特异性检测核酸 (NA) 靶标方面具有广泛的潜在应用。然而，这些方法中很少有进展到商业或临床应用。本综述总结了已知的 CRISPR/Cas 系统的特性及其应用，以及开发这些检测方法所面临的挑战，以及利用纳米/微技术平台提高其性能或解决未满足的检测需求的机会。这些机会包括快速高效的样品制备、集成单管、无扩增、可量化、多重和非 NA 检测。最后，本综述讨论了这些检测方法的现状，包括临床或即时检测应用以及其商业开发方面仍然存在的障碍。
