Kumar Tapsi, Nee Kevin, Wei Runmin, He Siyuan, Nguyen Quy H, Bai Shanshan, Blake Kerrigan, Gong Yanwen, Pein Maren, Sei Emi, Hu Min, Casasent Anna, Thennavan Aatish, Li Jianzhuo, Tran Tuan, Chen Ken, Nilges Benedikt, Kashikar Nachiket, Braubach Oliver, Cheikh Bassem Ben, Nikulina Nadya, Chen Hui, Teshome Mediget, Menegaz Brian, Javaid Huma, Nagi Chandandeep, Montalvan Jessica, Tifrea Delia F, Edwards Robert, Lin Erin, Parajuli Ritesh, Winocour Sebastian, Thompson Alastair, Lim Bora, Lawson Devon A, Kessenbrock Kai, Navin Nicholas
bioRxiv. 2023 Apr 25:2023.04.22.537946. doi: 10.1101/2023.04.22.537946.
The adult human breast comprises an intricate network of epithelial ducts and lobules that are embedded in connective and adipose tissue. While previous studies have mainly focused on the breast epithelial system, many of the non-epithelial cell types remain understudied. Here, we constructed a comprehensive Human Breast Cell Atlas (HBCA) at single-cell and spatial resolution. Our single-cell transcriptomics data profiled 535,941 cells from 62 women, and 120,024 nuclei from 20 women, identifying 11 major cell types and 53 cell states. These data revealed abundant pericyte, endothelial and immune cell populations, and highly diverse luminal epithelial cell states. Our spatial mapping using three technologies revealed an unexpectedly rich ecosystem of tissue-resident immune cells in the ducts and lobules, as well as distinct molecular differences between ductal and lobular regions. Collectively, these data provide an unprecedented reference of adult normal breast tissue for studying mammary biology and disease states such as breast cancer.
成年女性乳房由复杂的上皮导管和小叶网络组成，这些导管和小叶嵌入结缔组织和脂肪组织中。虽然先前的研究主要集中在乳腺上皮系统，但许多非上皮细胞类型仍未得到充分研究。在这里，我们以单细胞和空间分辨率构建了一个全面的人类乳腺细胞图谱（HBCA）。我们的单细胞转录组学数据分析了来自62名女性的535,941个细胞和来自20名女性的120,024个细胞核，确定了11种主要细胞类型和53种细胞状态。这些数据揭示了丰富的周细胞、内皮细胞和免疫细胞群体，以及高度多样的管腔上皮细胞状态。我们使用三种技术进行的空间映射揭示了导管和小叶中意想不到的丰富的组织驻留免疫细胞生态系统，以及导管和小叶区域之间明显的分子差异。总的来说，这些数据为研究乳腺生物学和乳腺癌等疾病状态提供了前所未有的成年正常乳腺组织参考。
Breast Cancer Res. 2014-3-28
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