Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
School of Medicine and Health Management, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
ACS Appl Mater Interfaces. 2023 May 24;15(20):24134-24148. doi: 10.1021/acsami.3c02646. Epub 2023 May 10.
In recent years, microbiota-based tumor immunotherapy has become a hotspot in cancer research. However, the use of microorganisms alone to activate the immune response for antitumor therapy was unsatisfactory. In this study, we biosynthesized gold nanoparticles (AuNPs) and platinum nanoparticles (PtNPs) based on yeast microcapsules to activate the immune response for antitumor treatment in synergy with chemodynamic therapy (CDT) and photothermal therapy (PTT). We generated AuNPs and PtNPs on yeast microcapsules (YAP) and fabricated nanoscale particles (Bre-YAP) by ultrasonic fragmentation and differential centrifugation. Bre-YAP retained the glucan component of yeast as an adjuvant; in the meantime, these two kinds of metal nanoparticles contained were excellent CDT and PTT mediators. By inspection, they could reach a high level of distribution in tumors and tumor-draining lymph nodes (TDLNs). Under the laser irradiation of tumors, this immunological nanomaterial significantly remodeled the microenvironments of tumors and TDLNs. The primary tumors were effectively inhibited or even eradicated, and the overall survival of mice was significantly improved as well. Therefore, yeast microcapsule-based Bre-YAP with immune properties could be used as an effective cancer treatment modality.
近年来，基于微生物组的肿瘤免疫疗法已成为癌症研究的热点。然而，单独使用微生物来激活免疫反应以进行抗肿瘤治疗的效果并不理想。在本研究中，我们基于酵母微囊泡生物合成了金纳米颗粒（AuNPs）和铂纳米颗粒（PtNPs），以协同化学动力学疗法（CDT）和光热疗法（PTT）激活免疫反应进行抗肿瘤治疗。我们在酵母微囊泡（YAP）上生成了 AuNPs 和 PtNPs，并通过超声破碎和差速离心制备了纳米级颗粒（Bre-YAP）。Bre-YAP 保留了酵母的葡聚糖成分作为佐剂；同时，这两种金属纳米颗粒都是出色的 CDT 和 PTT 介导物。经检查，它们可以在肿瘤和肿瘤引流淋巴结（TDLNs）中达到高水平分布。在肿瘤的激光照射下，这种免疫纳米材料显著重塑了肿瘤和 TDLNs 的微环境。有效抑制了原发肿瘤的生长，甚至将其消除，并且显著提高了小鼠的整体存活率。因此，具有免疫特性的基于酵母微囊泡的 Bre-YAP 可以作为一种有效的癌症治疗方式。
