Grupo de Sensores Químicos y Biosensores, Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Grupo de Sensores Químicos y Biosensores, Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049, Madrid, Spain; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Talanta. 2023 Aug 1;260:124614. doi: 10.1016/j.talanta.2023.124614. Epub 2023 May 5.
A novel immunosensor based on electrochemiluminescence resonance energy transfer (ECL-RET) for the sensitive determination of N protein of the SARS-CoV-2 coronavirus is described. For this purpose, bifunctional core@shell nanoparticles composed of a Pt-coated Au core and finally decorated with small Au inlays (Au@Pt/Au NPs) have been synthesized to act as ECL acceptor, using [Ru (bpy)] as ECL donor. These nanoparticles are efficient signaling probes in the immunosensor developed. The proposed ECL-RET immunosensor has a wide linear response to the concentration of N protein of the SARS-CoV-2 coronavirus with a detection limit of 1.27 pg/mL. Moreover, it has a high stability and shows no response to other proteins related to different virus. The immunosensor has achieved the quantification of N protein of the SARS-CoV-2 coronavirus in saliva samples. Results are consistent with those provided by a commercial colorimetric ELISA kit. Therefore, the developed immunosensor provides a feasible and reliable tool for early and effective detection of the virus to protect the population.
一种基于电化学发光共振能量转移（ECL-RET）的新型免疫传感器，用于灵敏测定 SARS-CoV-2 冠状病毒的 N 蛋白。为此，合成了具有 Pt 涂层 Au 核并最终用小的 Au 镶嵌物（Au@Pt/Au NPs）修饰的双功能核壳纳米粒子，作为 ECL 受体，使用 [Ru（bpy）] 作为 ECL 供体。这些纳米粒子是所开发的免疫传感器中的有效信号探针。所提出的 ECL-RET 免疫传感器对 SARS-CoV-2 冠状病毒 N 蛋白的浓度具有宽的线性响应，检测限为 1.27 pg/mL。此外，它具有高稳定性，对不同病毒相关的其他蛋白质没有响应。该免疫传感器已成功实现了唾液样本中 SARS-CoV-2 冠状病毒 N 蛋白的定量。结果与商业比色 ELISA 试剂盒提供的结果一致。因此，所开发的免疫传感器为早期和有效检测病毒提供了一种可行且可靠的工具，以保护人群。
Biosensors (Basel). 2024-2-18
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