State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Talanta. 2023 Aug 15;261:124672. doi: 10.1016/j.talanta.2023.124672. Epub 2023 May 13.
In this study, an electrochemiluminescence resonance energy transfer (ECL-RET) immunosensor was constructed to detect beta-2-microglobulin (B2M). As a donor-acceptor pair, a carbon nitride nanosheet modified with gold nanoparticles (Au@g-CNNSs) and a nickel- and iron-based organic framework modified with gold nanoparticles (Au@NiFeMOFs) were prepared. The sandwich immunosensor was successfully constructed so that ECL-RET occurred between Au@NiFeMOFs and Au@g-CNNSs. The ECL intensity of the immunosensor decreased with the increase the B2M concentration due to the low conductivity of B2M. The linear range of the ECL-RET immunosensor was from 10 fg/mL to 10 ng/mL, and the limit of detection was 2.3 fg/mL (S/N = 3). The developed immunosensor had high sensitivity, high specificity, and excellent stability. It could realize the sensitivity test of B2M and provide a novel idea for the detection of biomarkers.
在这项研究中，构建了一种用于检测β-2-微球蛋白（B2M）的电化学发光共振能量转移（ECL-RET）免疫传感器。作为供体-受体对，制备了负载金纳米粒子的氮化碳纳米片（Au@g-CNNSs）和负载金纳米粒子的镍铁基有机框架（Au@NiFeMOFs）。成功构建了三明治免疫传感器，使得 Au@NiFeMOFs 和 Au@g-CNNSs 之间发生 ECL-RET。由于 B2M 的低电导率，免疫传感器的 ECL 强度随着 B2M 浓度的增加而降低。ECL-RET 免疫传感器的线性范围为 10 fg/mL 至 10 ng/mL，检测限为 2.3 fg/mL（S/N = 3）。开发的免疫传感器具有高灵敏度、高特异性和优异的稳定性。它可以实现 B2M 的灵敏度测试，并为生物标志物的检测提供了新的思路。
