Kutateladze Institute of Thermophysics SB RAS, Novosibirsk, Russian Federation.
Novosibirsk State University, Novosibirsk, Russian Federation.
Biotechnol J. 2023 Aug;18(8):e2200644. doi: 10.1002/biot.202200644. Epub 2023 May 19.
Research into the flow structure in an aerial vortex bioreactor is relevant for developing the methods for growing cell cultures. Determining the optimal cultivation condition for a certain process is especially important in the case when such parameters of the medium as density and viscosity significantly change with the culture growth in the bioreactor.
The research of the flow dynamic was carried out in an 8.5 L universal aerial vortex bioreactor, with a washer freely floating on its surface and stabilizing the motion of the working fluid. The regularities of the vortex motion of the culture medium have been determined by Particle Image Velocimetry depending on its volume and the intensity of rotation of the activator, generating vortex motion in the air.
The observed vortex structure and its dynamics at increasing flow swirl intensity are established to coincide with the structure of a confined vortex flow in a cylindrical container with no washer for both single and two-fluid configurations. This novel methodology of the flow optimization shows that an ascending swirling jet forms in the vicinity of the bioreactor axis, and a bubble-like vortex breakdown forms in the axial region.
研究气升式涡旋生物反应器中的流动结构对于开发细胞培养方法具有重要意义。在生物反应器中，培养基的密度和粘度等参数随着培养物的生长而显著变化的情况下，确定特定过程的最佳培养条件尤为重要。
在 8.5L 通用气升式涡旋生物反应器中进行了流动动力学研究，在其表面自由浮动的洗涤器可稳定工作流体的运动。通过粒子图像测速法，根据培养基的体积和激活剂的旋转强度确定了培养基的涡旋运动规律，在空气中产生涡旋运动。
观察到的涡旋结构及其在增加流动旋转强度时的动力学与无洗涤器的圆柱形容器中受限涡旋流动的结构一致，无论是单流还是双流配置。这种新的流动优化方法表明，在生物反应器轴附近形成上升的旋流射流，在轴向区域形成气泡状的涡旋破裂。
