Sucosky Philippe, Osorio Diego F, Brown Jason B, Neitzel G Paul
Georgia Institute of Technology, The George W Woodruff School of Mechanical Engineering, 771 Ferst Drive, Atlanta, Georgia 30332-0405, USA.
Biotechnol Bioeng. 2004 Jan 5;85(1):34-46. doi: 10.1002/bit.10788.
Spinner-flask bioreactors have been used for the production of articular cartilage in vitro. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. The present research focuses on the experimental and numerical characterization of the flow field within a spinner flask operating under conditions used to produce cartilage. Laboratory experiments carried out in a scaled-up model bioreactor employ particle-image velocimetry (PIV) to determine velocity and shear-rate fields in the vicinity of the construct closest to the stir bar, in addition to turbulence properties. Numerical computations calculated using FLUENT, a commercial software package, simulate the flow field in the same model bioreactor under similar operating conditions. In the computations, scaffolds were modeled as both solid and porous media with different permeabilities and flow rates through various faces of the construct nearest the stir bar were examined.
转瓶生物反应器已被用于体外生产关节软骨。已知生物反应器内的动态环境会显著影响组织的生长和发育。本研究聚焦于在用于生产软骨的条件下运行的转瓶内流场的实验和数值表征。在放大的模型生物反应器中进行的实验室实验采用粒子图像测速技术（PIV）来确定最靠近搅拌棒的构建体附近的速度和剪切速率场，以及湍流特性。使用商业软件包FLUENT进行的数值计算模拟了同一模型生物反应器在相似运行条件下的流场。在计算中，支架被建模为具有不同渗透率的固体和多孔介质，并研究了通过最靠近搅拌棒的构建体各面的流速。
