Schubert Rudolf, Gaynullina Dina, Shvetsova Anastasia, Tarasova Olga S
Physiology, Institute of Theoretical Medicine, Faculty of Medicine, University of Augsburg, Augsburg, Germany.
Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
Front Physiol. 2023 Apr 24;14:1176748. doi: 10.3389/fphys.2023.1176748. eCollection 2023.
The study of the mechanisms of regulation of vascular tone is an urgent task of modern science, since diseases of the cardiovascular system remain the main cause of reduction in the quality of life and mortality of the population. Myography (isometric and isobaric) of isolated blood vessels is one of the most physiologically relevant approaches to study the function of cells in the vessel wall. On the one hand, cell-cell interactions as well as mechanical stretch of the vessel wall remain preserved in myography studies, in contrast to studies on isolated cells, e.g., cell culture. On the other hand, studies in isolated vessels allow control of numerous parameters that are difficult to control . The aim of this review was to 1) discuss the specifics of experimental design and interpretation of data obtained by myography and 2) highlight the importance of the combined use of myography with various complementary techniques necessary for a deep understanding of vascular physiology.
血管张力调节机制的研究是现代科学的一项紧迫任务，因为心血管系统疾病仍然是降低人口生活质量和导致人口死亡的主要原因。离体血管的肌动描记法（等长和等压）是研究血管壁细胞功能最符合生理学的方法之一。一方面，与例如细胞培养等离体细胞研究不同，在肌动描记法研究中，细胞间相互作用以及血管壁的机械拉伸得以保留。另一方面，离体血管研究允许控制许多难以控制的参数。本综述的目的是：1）讨论肌动描记法实验设计的细节以及对所获数据的解读，2）强调肌动描记法与各种补充技术联合使用对于深入理解血管生理学的重要性。
