Potekhina Ramziya M, Tarasova Evgenya Yu, Matrosova Lilia E, Khammadov Nail I, Saifutdinov Alexander M, Ermolaeva Olga K, Tanaseva Svetlana A, Mishina Nailya N, Nigmatulin Gali N, Mukharlyamova Aisylu Z, Smolentsev Sergey Yu, Semenov Eduard I
Federal Center for Toxicological, Radiation and Biological Safety, Kazan 420075, Nauchnyi Gorodok-2, Russia.
Mari State University, Lenin Square 1, Yoshkar-Ola City 424000, Russia.
Vet Med Int. 2023 Apr 30;2023:5281260. doi: 10.1155/2023/5281260. eCollection 2023.
In this article, we present the first case report of a chicken mycosis caused by occurred on a private farm in the Russian Federation. Lesions on the skin of the legs and scallops were reported. The object of this study was samples of feed and pathological material from sick hens-layers. Mycological analysis included determination of the total number of fungi (TNF) and identification and determination of the toxicity and pathogenicity of the isolates. The identification of the isolate was carried out taking into account direct microscopy, morphological features, and the method of molecular genetic analysis. Microscopic fungi of the genus and were isolated by mycological analysis of the feed. The test feed was nontoxic. Mycological examination of pathological material (scrapings from the combs and affected legs) identified an isolate of , which showed toxicity on biological objects (protozoa, rabbits) and pathogenicity (white mice). Dermal application of suspension was accompanied by reddening of the rabbit skin. Intraperitoneal injection of fungal spores caused mycosis in white mice. Polymerase chain reaction (PCR) made it possible to identify this type of microscopic fungus () with high accuracy in the samples under study. The research results allow us to consider as a cause of poultry disease against the background of predisposing factors in the form of desquamation of the stratum corneum of the skin against the background of immunosuppression and metabolic disorders caused by an imbalance in the diet.
在本文中,我们报告了俄罗斯联邦一个私人农场发生的首例由[未提及具体真菌名称]引起的鸡霉菌病病例。报告了腿部皮肤和肉垂出现病变。本研究的对象是患病蛋鸡的饲料样本和病理材料。真菌学分析包括测定真菌总数(TNF)以及对分离株的毒性和致病性进行鉴定和测定。分离株的鉴定是综合直接显微镜检查、形态特征以及分子遗传分析方法进行的。通过对饲料的真菌学分析分离出了[未提及具体真菌属名]属的显微镜下可见真菌。受试饲料无毒。对病理材料(鸡冠和患病腿部的刮屑)进行真菌学检查,鉴定出一种[未提及具体真菌名称]的分离株,该分离株对生物对象(原生动物、兔子)显示出毒性,对(白色小鼠)具有致病性。给兔子皮肤涂抹[未提及具体真菌名称]悬浮液后皮肤发红。给白色小鼠腹腔注射真菌孢子导致其感染霉菌病。聚合酶链反应(PCR)能够在研究样本中高精度地鉴定出这种显微镜下可见真菌([未提及具体真菌名称])。研究结果使我们能够认为,在免疫抑制和因饮食不平衡导致代谢紊乱背景下皮肤角质层脱屑这一易感因素的背景下,[未提及具体真菌名称]是家禽疾病的一个病因。