Zhao Limin, Xie Xiuchao, Chen Dejing, Ma Guilan, Sun Ya
Shaanxi University of Technology, College of Bioscience and Engineering / Shaanxi Key-Laboratory of Bioresources, #1, East 1st Ring Road, Hanzhong, 723001, Shaanxi, China.
BMC Zool. 2022 Jun 6;7(1):31. doi: 10.1186/s40850-022-00123-y.
The worm Enchytraeus buchholzi is a new record species for Shaanxi, China, and a key pest on American ginseng Panax quinquefolium. To distinguish the species, the authors prepared its whole mounts and paraffin-embedded sections, and microscopically observed, photographed and measured. Besides, we conducted an experimental study on its DNA barcode. RESULTS: Cells, tissues and organs related to nervous, digestive, circulatory, excretory and reproductive systems were found, photomicrographed and described, including: prostomium, peristomium, segments, clitellum, pygidium, lateral and ventral chaetae; brain, cranial nerves, sensory papillae, ventral nerve cord; pharyngeal pad and glands, retractor muscles and muscular bundles, peptonephridia, esophagus, intestine; dorsal, lateral, ventral and intestinal parietal vessels, coelomocytes, coelomic cavity; nephridia, chloragogen cells; ovaries, groups of germ cells with developing oocytes, mature eggs, spermathecae; testes, seminal vesicles, sperm funnels, penial bulbs. Their shapes and sizes were given, and functions discussed briefly. The visual effect of staining specimens with hematoxylin plus eosin ranked the first, and that with acetocarmine the second. CONCLUSIONS: The supplementary and objective descriptions, with the microphotographs as forceful pieces of evidence, have expanded biological knowledge in aspects of the form, structure and function of the worm, which is helpful for professionals to recognize and understand this species and provide a solid basis for its integrated pest management.
蚯蚓巴氏线蚓是中国陕西新记录物种，也是西洋参的一种关键害虫。为鉴别该物种，作者制作了其整体装片和石蜡切片，并进行显微镜观察、拍照和测量。此外，我们对其DNA条形码进行了实验研究。结果：发现了与神经、消化、循环、排泄和生殖系统相关的细胞、组织和器官，并进行了显微拍照和描述，包括：口前叶、围口节、体节、环带、尾节、侧刚毛和腹刚毛；脑、脑神经、感觉乳突、腹神经索；咽垫和腺体、牵引肌和肌束、消化肾管、食管、肠；背血管、侧血管、腹血管和肠壁血管、体腔细胞、体腔；肾管、黄色细胞；卵巢、有发育中卵母细胞的生殖细胞群、成熟卵、受精囊；精巢、精囊、精子漏斗、阴茎球。给出了它们的形状和大小，并简要讨论了功能。苏木精加伊红染色标本的视觉效果最佳，醋酸洋红染色标本次之。结论：以显微照片为有力证据的补充性和客观性描述，扩展了关于该蚯蚓形态、结构和功能方面的生物学知识，有助于专业人员识别和了解该物种，并为其害虫综合治理提供坚实基础。
