Department of Physics, Collaborative Innovation Center of Advanced Microstructures, MOE Key Laboratory of Modern Acoustics, Nanjing University, Nanjing, China.
Department of Ultrasound, Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.
J Biophotonics. 2023 Aug;16(8):e202300058. doi: 10.1002/jbio.202300058. Epub 2023 May 17.
Microcirculation imaging has significantly clinical value in early diagnosis and curative effect judgment of various diseases. The most superficial layer of the nailbed is rich in capillaries, which is suitable as a window on the microcirculation. However, few techniques can noninvasively observe the blood supply distribution of the nailbed, especially for high-resolution imaging of capillaries. In this study, we adapted an optical-resolution photoacoustic microscopy (OR-PAM) to image the nailbed microvasculature. The imaging sensitivity was significantly improved by hydration pretreatment of the nail. In vitro phantom experiments demonstrate that the sensitivity was improved about 3.5 times after hydration. In vivo imaging experiments of the nailbed microvasculature were conducted to further examine the enhanced sensitivity and practicability of OR-PAM. Moreover, the quantitative analysis of capillary loops showed that OR-PAM can extract the detection indicators including vascular morphology, diameter, and length, which provides a basis for clinical microcirculation detection using OR-PAM.
微血管成像在各种疾病的早期诊断和疗效判断方面具有重要的临床价值。甲床的最浅层富含毛细血管，是观察微循环的理想窗口。然而，很少有技术可以非侵入性地观察甲床的血液供应分布，特别是对于毛细血管的高分辨率成像。在本研究中，我们采用光学分辨率光声显微镜（OR-PAM）来对甲床微血管成像。通过对指甲进行水合预处理，显著提高了成像灵敏度。体外仿体实验表明，水合后灵敏度提高了约 3.5 倍。对甲床微血管进行的体内成像实验进一步检验了 OR-PAM 的增强灵敏度和实用性。此外，对毛细血管环的定量分析表明，OR-PAM 可以提取包括血管形态、直径和长度在内的检测指标，为使用 OR-PAM 进行临床微循环检测提供了依据。
