Wolff N A, Perlman D F, Goldstein L
Am J Physiol. 1986 Jun;250(6 Pt 2):R984-90. doi: 10.1152/ajpregu.1986.250.6.R984.
A peritubular mechanism for active taurine uptake, similar to that previously found in the flounder, was demonstrated in killifish (Fundulus heteroclitus) teased renal tubules. Other beta-amino acids and glycine inhibited taurine transport. Uptake exhibited a nearly absolute requirement for external Na+. The stoichiometric relationship between Na+ and taurine was found to be 2:1. Medium Cl- markedly stimulated the Na+-dependent taurine uptake, but none of the other small monovalent anions tested (NO3-, Br-, SCN-) could substitute for Cl- in activating taurine transport. Cl- replacement resulted in a significant decrease in V max, whereas the affinity of the carrier for taurine appeared to be unaffected. In the absence of Cl- the Na+ dependence of taurine influx was reduced to a stoichiometric relationship of 1.4 Na+:1 taurine, indicating an effect of Cl- on the binding of Na+ to the transport system. A model for Na+ -Cl- -taurine cotransport is presented.
在将鳉鱼（Fundulus heteroclitus）的肾小管分离出来进行研究时，发现了一种类似于之前在比目鱼中发现的、用于主动摄取牛磺酸的肾小管周围机制。其他β-氨基酸和甘氨酸会抑制牛磺酸的转运。摄取过程对细胞外Na+几乎有绝对的需求。发现Na+与牛磺酸的化学计量关系为2:1。培养基中的Cl-显著刺激了依赖Na+的牛磺酸摄取，但测试的其他单价小阴离子（NO3-、Br-、SCN-）均不能替代Cl-来激活牛磺酸转运。用其他离子替代Cl-会导致Vmax显著降低，而载体对牛磺酸的亲和力似乎未受影响。在没有Cl-的情况下，牛磺酸内流对Na+的依赖性降低至1.4个Na+与1个牛磺酸的化学计量关系，这表明Cl-对Na+与转运系统结合有影响。本文提出了一个Na+-Cl--牛磺酸共转运模型。
