Zhou Zhiqiang, Xu Xinyi, Luo Dongmei, Zhou Zhiwei, Zhang Senlin, He Ruipeng, An Tianwu, Sun Qun
College of Biomass Science and Engineering, Sichuan University, Chengdu 610064, China.
Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
Foods. 2023 Apr 29;12(9):1858. doi: 10.3390/foods12091858.
Probiotics have gained tremendous attention as an alternative to antibiotics, while synbiotics may exhibit a greater growth promoting effect than their counterpart probiotics due to the prebiotics' promotion on the growth and reproduction of probiotics. The objective of this study was to investigate the influence of N-1 and its synbiotic with oligomeric isomaltose on the growth performance and meat quality of Hu sheep. Hu sheep (0-3 days old) were fed with water, probiotics of N-1, or synbiotics (N-1 and oligomeric isomaltose) daily in three pens for 60 days and regularly evaluated to measure growth performance and collect serum (five lambs per group). (LT) and (BB) muscle tissues were collected for the analysis of pH value, color, texture, nutrients, mineral elements, amino acids, volatile compounds, and antioxidant capacity. The results showed that dietary supplementation of N-1 tended to improve growth performance and meat quality of Hu sheep, while the synergism of N-1 with oligomeric isomaltose significantly improved their growth performance and meat quality ( < 0.05). Both the dietary supplementation of N-1 and synbiotics ( < 0.05) increased the body weight and body size of Hu sheep. Synbiotic treatment reduced serum cholesterol and improved LT fat content by increasing the transcription level of fatty acid synthase to enhance fat deposition in LT, as determined via RT-qPCR analysis. Moreover, synbiotics increased zinc content and improved LT tenderness by decreasing shear force and significantly increased the levels of certain essential (Thr, Phe, and Met) and non-essential (Asp, Ser, and Tyr) amino acids of LT ( < 0.05). Additionally, synbiotics inhibited the production of carbonyl groups and TBARS in LT and thus maintained antioxidant stability. In conclusion, it is recommended that the use of synbiotics in livestock breeding be promoted to improve sheep production and meat quality.
益生菌作为抗生素的替代品已受到极大关注，而合生元由于益生元对益生菌生长和繁殖的促进作用，可能比其对应的益生菌表现出更大的促生长效果。本研究的目的是探讨N-1及其与低聚异麦芽糖的合生元对湖羊生长性能和肉质的影响。将0至3日龄的湖羊分三组每天分别喂水、N-1益生菌或合生元（N-1和低聚异麦芽糖），共60天，并定期评估以测定生长性能并采集血清（每组五只羔羊）。采集（LT）和（BB）肌肉组织用于分析pH值、颜色、质地、营养成分、矿物元素、氨基酸、挥发性化合物和抗氧化能力。结果表明，日粮添加N-1有改善湖羊生长性能和肉质的趋势，而N-1与低聚异麦芽糖的协同作用显著改善了它们的生长性能和肉质（P<0.05）。日粮添加N-1和合生元均增加了湖羊的体重和体尺（P<0.05）。通过RT-qPCR分析确定，合生元处理降低了血清胆固醇，并通过提高脂肪酸合酶的转录水平增加了LT脂肪含量，从而改善了LT脂肪沉积。此外，合生元增加了锌含量，通过降低剪切力改善了LT嫩度，并显著提高了LT中某些必需氨基酸（苏氨酸、苯丙氨酸和蛋氨酸）和非必需氨基酸（天冬氨酸、丝氨酸和酪氨酸）的水平（P<0.05）。此外，合生元抑制了LT中羰基和TBARS的产生，从而维持了抗氧化稳定性。总之，建议在畜牧养殖中推广使用合生元，以提高绵羊生产性能和肉质。
