Cui Leihong, Zeng Hui, Hou Meixin, Li Zhongxin, Mu Chunlong, Zhu Weiyun, Hang Suqin
National Center for International Research on Animal Gut Nutrition, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China.
Anim Nutr. 2023 Jul 3;14:370-382. doi: 10.1016/j.aninu.2023.06.008. eCollection 2023 Sep.
Alternatives to antibiotics for preventing bacteria-induced inflammation in early-weaned farm animals are sorely needed. Our previous study showed that L47 and inulin could alleviate dextran sulfate sodium (DSS)-induced colitis in mice. To explore the protective effects of L47 and inulin on the ileal inflammatory response in weaned piglets challenged with enterotoxigenic (ETEC), 28 weaned piglets were assigned into four groups, namely, CON group-orally given 10 mL/d phosphate buffer saline (PBS), LI47 group-orally given a mixture of 10 mL/d L47 and inulin, ECON group-orally given 10 mL/d PBS and challenged by ETEC, and ELI47 group-orally given 10 mL/d L47 and inulin mixture and challenged by ETEC. The results demonstrated that the combination of L47 and inulin reduced inflammatory responses and relieved the inflammatory damage caused by ETEC, including ileal morphological damage, reduced protein expression of ileal tight junction, decreased antioxidant capacity, and decreased anti-inflammatory factors. Transcriptome analysis revealed that L47 and inulin up-regulated the gene expression of phospholipase A2 group IIA (PLA2G2A) ( < 0.05) as well as affected alpha-linolenic acid (ALA) metabolism and linoleic acid metabolism. Moreover, L47 and inulin increased the levels of ALA ( < 0.05), lipoteichoic acid (LTA) ( < 0.05), and 12,13-epoxyoctadecenoic acid (12,13-EpOME) ( < 0.05) and the protein expression of Toll-like receptor 2 (TLR2) ( = 0.05) in the ileal mucosa. In conclusion, L47 and inulin together alleviated ETEC-induced ileal inflammation in piglets by up-regulating the levels of ALA and 12,13-EpOME via the LTA/TLR2/PLA2G2A pathway.
迫切需要抗生素的替代物来预防早期断奶农场动物的细菌感染性炎症。我们之前的研究表明，L47和菊粉可以减轻葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎。为了探究L47和菊粉对产肠毒素大肠杆菌（ETEC）攻击的断奶仔猪回肠炎症反应的保护作用，将28头断奶仔猪分为四组，即CON组——每天口服10 mL磷酸盐缓冲盐水（PBS），LI47组——每天口服10 mL L47和菊粉的混合物，ECON组——每天口服10 mL PBS并接受ETEC攻击，ELI47组——每天口服10 mL L47和菊粉的混合物并接受ETEC攻击。结果表明，L47和菊粉的组合降低了炎症反应，减轻了ETEC引起的炎症损伤，包括回肠形态损伤、回肠紧密连接蛋白表达降低、抗氧化能力下降和抗炎因子减少。转录组分析显示，L47和菊粉上调了磷脂酶A2第二亚家族A（PLA2G2A）的基因表达（P<0.05），并影响了α-亚麻酸（ALA）代谢和亚油酸代谢。此外，L47和菊粉增加了回肠黏膜中ALA（P<0.05）、脂磷壁酸（LTA）（P<0.05）和12,13-环氧十八碳烯酸（12,13-EpOME）（P<0.05）的水平以及Toll样受体2（TLR2）的蛋白表达（P=0.05）。总之，L47和菊粉共同通过LTA/TLR2/PLA2G2A途径上调ALA和12,13-EpOME的水平，减轻了ETEC诱导的仔猪回肠炎症。
