Di Luca Alessio, Ianni Andrea, Bennato Francesca, Martino Camillo, Henry Michael, Meleady Paula, Martino Giuseppe
Department of Bioscience and Technology for Food Agro-Food and Environmental Technology, University of Teramo, 64100 Teramo, Italy.
Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
Animals (Basel). 2023 Apr 25;13(9):1460. doi: 10.3390/ani13091460.
Capital-driven animal husbandry systems undertaken in the last century led to the abandoning of many pig breeds that were not profitable. These local pig breeds and their respective production systems have great potential as they are able to respond to the high criteria and needs of modern society concerning some environmental aspects, animal-welfare, healthiness, etc. This is the case of the black pigs of Italy. The Apulo-Calabrese is a breed of black pig, known by many other names such as Nero d'Abruzzo. In order to further understand the biological differences between different types of porcine genetics (Nero d'Abruzzo and commercial-hybrid) we used a label-free LC-MS strategy and Western-blot to characterize the proteomes of muscle-exudate collected from these pigs. This proteomics approach identified 1669 proteins of which 100 changed significantly in abundance between breeds. Bioinformatics functional analysis indicated that differentially expressed proteins were involved in several biological processes related to energy-metabolism and response to oxidative stress, suggesting that these functions might distinguish between these pigs. Fatty-acid synthase, catalase and glutathione-peroxidase, involved in enzymatic activity were found to be more represented in samples obtained from the Nero d'Abruzzo. This biological information can potentially provide new biological factors that could determine the different production performances of these pigs, distinguished by their different genetic backgrounds.
上世纪推行的资本驱动型畜牧业体系导致许多无利可图的猪品种被淘汰。这些本地猪品种及其各自的生产系统具有巨大潜力，因为它们能够满足现代社会在某些环境方面、动物福利、健康等方面的高标准和需求。意大利黑猪就是如此。阿普利亚 - 卡拉布里亚猪是一种黑猪品种，还有许多其他名称，如阿布鲁佐黑猪。为了进一步了解不同类型猪遗传（阿布鲁佐黑猪和商业杂交猪）之间的生物学差异，我们采用了无标记液相色谱 - 质谱策略和蛋白质免疫印迹法来表征从这些猪采集的肌肉渗出液的蛋白质组。这种蛋白质组学方法鉴定出1669种蛋白质，其中100种在品种间丰度有显著变化。生物信息学功能分析表明，差异表达的蛋白质参与了与能量代谢和氧化应激反应相关的几个生物学过程，这表明这些功能可能区分这些猪。参与酶活性的脂肪酸合酶、过氧化氢酶和谷胱甘肽过氧化物酶在从阿布鲁佐黑猪获得的样本中含量更高。这些生物学信息可能会提供新的生物学因素，这些因素可以决定这些具有不同遗传背景的猪的不同生产性能。
