Department of Agricultural and Forestry Engineering, ETSIIAA, Universidad de Valladolid, Avda. Madrid 44, 34004 Palencia, Spain.
Department of Plant Science, Agrifood Research and Technology Centre of Aragón, Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Avda. Montañana 930, 50059 Zaragoza, Spain.
Molecules. 2023 Apr 26;28(9):3730. doi: 10.3390/molecules28093730.
The cliff rose (), like other halophytes, has a phenolics-based antioxidant system that allows it to grow in saline habitats. Provided that antioxidant properties are usually accompanied by antimicrobial activity, in this study we investigated the phytochemicals present in a hydromethanolic extract of flowers and explored its antifungal potential. The main phytocompounds, identified by gas chromatography-mass spectrometry, were: hexadecanoic acid, octadecanoic acid, 9-octadecenoic acid, 3-(3,4-dihydroxy-phenyl)-acrylic acid ethyl ester, and benzeneacetaldehyde. The antifungal activity of the extract and its main constituents-alone and in combination with chitosan oligomers-was tested against six pathogenic taxa associated with soil-borne diseases of plant hosts in the family Cucurbitaceae: , f. sp. , , , , and . In in vitro tests, EC effective concentrations in the 166-865 μg·mL range were obtained for the chitosan oligomers- extract conjugate complexes, lower than those obtained for fosetyl-Al and azoxystrobin synthetic fungicides tested for comparison purposes, and even outperforming mancozeb against . In ex situ tests against conducted on artificially inoculated cucumber slices, full protection was achieved at a dose of 250 μg·mL. Thus, the reported results support the valorization of as a source of biorationals for Cucurbitaceae pathogens protection, suitable for both organic and conventional agriculture.
悬崖柳（）与其他盐生植物一样，具有基于酚类的抗氧化系统，使其能够在盐生环境中生长。鉴于抗氧化特性通常伴随着抗菌活性，本研究调查了水-甲醇提取物中存在的植物化学物质，并探索了其抗真菌潜力。通过气相色谱-质谱联用鉴定的主要植物化合物为：十六烷酸、十八烷酸、9-十八烯酸、3-（3,4-二羟基-苯基）-丙烯酸乙酯和苯乙醛。提取物及其主要成分——单独使用和与壳寡糖结合使用——的抗真菌活性针对与葫芦科植物土传病害相关的六个病原物进行了测试：、、、、和。在体外试验中，壳寡糖-提取物缀合物复合物的 EC 有效浓度在 166-865 μg·mL 范围内，低于为比较目的测试的福美双和肟菌酯合成杀菌剂的 EC 有效浓度，甚至优于代森锰锌对的效果。在对人工接种的黄瓜片进行的原位试验中，在 250 μg·mL 的剂量下可实现完全保护。因此，报告的结果支持将作为防治葫芦科病原菌的生物制剂的来源加以利用，适用于有机和常规农业。
