School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.
Key Laboratory of Colloid and Interface Chemistry (Ministry of Education), School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
Molecules. 2023 May 6;28(9):3929. doi: 10.3390/molecules28093929.
The burgeoning need to study the applications of nanoparticles (NPs) in biomedical and pharmaceutical fields requires an understanding of their interactions with lipid membranes for further in vivo studies. In this paper, negatively charged egg yolk lecithin liposome (EYL) has been prepared and used as model lipid membranes. Positively charged MgAl-layered double hydroxides (LDHs) are viewed as models of clay particles. The ability of the LDH NPs, a two-dimensional nanostructure with an average diameter of 100 nm (LDHs-100) or 500 nm (LDHs-500) to cross the membranes, has been thoroughly investigated via (high-resolution) transmission electron microscopy (TEM), optical microscopy (OM), scanning electron microscopy (SEM), confocal fluorescence microscopy (CLSM), and dynamic light scattering (DLS). The liposomes with an average diameter of 1.5 μm were prepared by the thin-film rehydration method followed by an extrusion technique. A calcein leakage assay and steady-state fluorescence measurement displayed the variation of membrane integrity and polarity of the pyrene-located microenvironment during the interaction between EYL and calcein-interacted LDH NPs (CE-LDHs) or LDH NPs, respectively. These results imply that not only spherical particles but also even more sophisticated nanostructured materials are able to effectively cross the lipid bilayers, thereby engineering new compounds that may be encapsulated for safe and potential use in biomedical applications.
由于需要研究纳米粒子（NPs）在生物医学和制药领域的应用，因此需要了解它们与脂质膜的相互作用，以便进一步进行体内研究。在本文中，我们制备了带负电荷的卵黄磷脂囊泡（EYL）作为模型脂质膜，并使用带正电荷的 MgAl 层状双氢氧化物（LDHs）作为粘土颗粒的模型。通过（高分辨率）透射电子显微镜（TEM）、光学显微镜（OM）、扫描电子显微镜（SEM）、共聚焦荧光显微镜（CLSM）和动态光散射（DLS），我们深入研究了平均直径为 100nm（LDHs-100）或 500nm（LDHs-500）的二维纳米结构 LDHs NPs 穿过细胞膜的能力。通过薄膜再水化法制备平均直径为 1.5μm 的脂质体，然后通过挤出技术进一步处理。钙黄绿素渗漏实验和稳态荧光测量显示了在 EYL 与钙黄绿素相互作用的 LDHs NPs（CE-LDHs）或 LDHs NPs 相互作用过程中，膜完整性和位于芘微环境极性的变化。这些结果表明，不仅球形颗粒，甚至更复杂的纳米结构材料也能够有效地穿过脂质双层，从而设计出新的化合物，这些化合物可能被封装用于安全且有潜力的生物医学应用。
