Research Department, Srebrnjak Children's Hospital, 10000 Zagreb, Croatia.
Department of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, Croatia.
Int J Mol Sci. 2023 Apr 22;24(9):7698. doi: 10.3390/ijms24097698.
The proportion of oral and oropharyngeal squamous cell carcinoma (OOSCC) that can be attributed to human papillomavirus (HPV) infection is growing nowadays. A potential factor indicating the occurrence of HPV-positive OSCC is a change in the degree of methylation of gene promoters that play a key role in the immune response. In this study, we investigated the difference in the methylation of , , , , , , and gene promoters in samples of healthy oral mucosa versus samples of oral and oropharyngeal cancer. The presence of HPV infection in samples was examined earlier. To determine the difference in methylation of those gene promotors, isolated and bisulfite-modified DNA was analysed by the methylation-specific PCR method. The investigated gene promoters were found to be more hypomethylated in the oral and oropharyngeal cancer samples in comparison to normal tissue. The proportion of unmethylated gene promoters was similar in HPV-positive and HPV-negative cancers, although the data should be confirmed on a larger set of samples. To conclude, in samples of healthy oral mucosa, the investigated gene promoters were found to be methylated in a high percentage (73.3% to 100%), while in oral and oropharyngeal cancer samples, they were methylated in a low percentage (11.1% to 37%), regardless of HPV infection.
目前，可归因于人类乳头瘤病毒（HPV）感染的口腔和口咽鳞状细胞癌（OOSCC）比例正在上升。HPV 阳性 OSCC 发生的一个潜在因素是在基因启动子的甲基化程度上发生变化，这些基因启动子在免疫反应中起着关键作用。在这项研究中，我们研究了健康口腔黏膜样本与口腔和口咽癌样本中 、 、 、 、 、 和 基因启动子甲基化的差异。之前检查了样本中 HPV 感染的存在。为了确定这些基因启动子的甲基化差异，通过甲基化特异性 PCR 方法分析了分离并经亚硫酸氢盐修饰的 DNA。与正常组织相比，在口腔和口咽癌样本中，所研究的基因启动子被发现低甲基化。尽管应该在更大的样本集上确认这些数据，但 HPV 阳性和 HPV 阴性癌症中未甲基化基因启动子的比例相似。总之，在健康口腔黏膜样本中，所研究的基因启动子被发现高度甲基化（73.3%至 100%），而在口腔和口咽癌样本中，无论 HPV 感染如何，它们的甲基化程度都较低（11.1%至 37%）。
