使用磷酸4-甲基伞形酮对钙调蛋白依赖性蛋白磷酸酶进行荧光分光光度测定。
A spectrofluorimetric assay of calmodulin-dependent protein phosphatase using 4-methylumbelliferyl phosphate.
作者信息
Anthony F A, Merat D L, Cheung W Y
出版信息
Anal Biochem. 1986 May 15;155(1):103-7. doi: 10.1016/0003-2697(86)90232-0.
PMID:3717545
Abstract
A continuous spectrofluorimetric assay of calmodulin-dependent phosphatase is described. The assay monitors the formation of a fluorescent 4-methylumbelliferone from the dephosphorylation of 4-methylumbelliferyl phosphate and detects as little as 1 pmol of 4-methylumbelliferone. The phosphatase shows a Km of 1.3 mM for the substrate and a Vmax of 100 nmol/mg/min.
摘要
本文描述了一种对钙调蛋白依赖性磷酸酶的连续荧光分光光度测定法。该测定法监测由磷酸4-甲基伞形酮去磷酸化形成荧光4-甲基伞形酮的过程，并且能够检测低至1皮摩尔的4-甲基伞形酮。该磷酸酶对底物的米氏常数（Km）为1.3 mM，最大反应速度（Vmax）为100 nmol/mg/min。
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