H. Buniatian Institute of Biochemistry of National Academy of Sciences, 5/1 P. Sevag Str, Yerevan, 0014, Republic of Armenia.
Neurochem Res. 2010 Jun;35(6):925-33. doi: 10.1007/s11064-009-0097-3. Epub 2009 Dec 5.
The effect of synthetic LVV-hemorphin-7 and hemorphin-7 on hypothalamo-pituitary-adrenocortical axis activity in response to endotoxin-induced stress was studied. The intraperitoneal (ip) endotoxin (lipopolysaccaride, LPS) (0.5 mg/kg) administration in combination with hemorphin (1 mg/kg) induce significant decrease in plasma corticosterone and modest decrease in plasma levels of tumor necrosis factor-alpha (TNFalpha) in compare with elevated levels of both corticosterone and TNFalpha in plasma of rats received LPS administration alone. Increased activity of calcineurin in both plasma and brain of rats received ip administration of LPS, was recovered under LPS + hemorphin treatment. In two independent proteome analysis, using 2-dimensional fluorescence difference gel electrophoresis and the isotope coded protein label technology, peptidyl-prolyl cis-trans-isomerase A (cyclophilin A) was identified as regulated by hemorphins protein in mouse brain. A therapeutic potential of hemorphins and mechanisms of their homeostatic action in response to endotoxin-induced stress are discussed.
研究了合成 LVV-hemorphin-7 和 hemorphin-7 对脂多糖（LPS）诱导的应激下丘脑-垂体-肾上腺皮质轴活动的影响。与单独给予 LPS 组相比，腹腔内给予 LPS（0.5mg/kg）与 hemorphin（1mg/kg）联合给药可显著降低血浆皮质酮水平，并适度降低肿瘤坏死因子-α（TNFalpha）水平。在单独给予 LPS 的大鼠的血浆和大脑中，钙调神经磷酸酶的活性增加，在 LPS + hemorphin 处理下恢复。在使用二维荧光差异凝胶电泳和同位素编码蛋白质标签技术的两项独立蛋白质组分析中，发现肽基脯氨酰顺反异构酶 A（亲环素 A）被 hemorphins 蛋白调节。讨论了 hemorphins 的治疗潜力及其在应对内毒素诱导的应激中的稳态作用机制。
