使用4-甲基伞形酮基酪蛋白测定蛋白酶活性的荧光法。
Fluorigenic method for the assay of proteinase activity with the use of 4-methylumbelliferyl-casein.
作者信息
Khalfan H, Abuknesha R, Robinson D
出版信息
Biochem J. 1983 Jan 1;209(1):265-7. doi: 10.1042/bj2090265.
Abstract
A method for the preparation of casein labelled with the 4-methylumbelliferyl fluorophore is described, and the product was used as a fluorigenic macromolecular substrate for a sensitive assay of the activity of proteinases. Nanogram quantities of trypsin, chymotrypsin, elastase and cathepsin D can be detected, but the substrate is unaffected by cathepsin B.
摘要
描述了一种制备用4-甲基伞形酮荧光团标记的酪蛋白的方法，该产物用作荧光大分子底物，用于灵敏测定蛋白酶的活性。可以检测到纳克量的胰蛋白酶、胰凝乳蛋白酶、弹性蛋白酶和组织蛋白酶D，但该底物不受组织蛋白酶B的影响。
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