Barker S A, Hsieh L C, Short C R
Anal Biochem. 1986 May 15;155(1):112-8. doi: 10.1016/0003-2697(86)90234-4.
New methodology for the extraction and analysis of the anthelmintic fenbendazole and its metabolites from plasma, urine, liver homogenates, and feces from several animal species is presented. Quantitation of fenbendazole and its metabolites was conducted by high-pressure liquid chromatography using ultraviolet detection at 290 nm. The combined extraction and analysis procedures give excellent recoveries in all of the different biological matrices examined. High specificity, low limits of detection, and excellent linearity, accuracy, and inter- and intrasample variability were also obtained. The study of fenbendazole pharmacokinetics in vitro and in vivo should be greatly enhanced through the utilization of these methods.
本文介绍了一种从几种动物的血浆、尿液、肝脏匀浆和粪便中提取和分析驱虫药芬苯达唑及其代谢物的新方法。芬苯达唑及其代谢物的定量分析采用高压液相色谱法，在290nm波长下进行紫外检测。在所有检测的不同生物基质中，联合提取和分析程序均具有出色的回收率。同时还获得了高特异性、低检测限以及出色的线性、准确性和样品间及样品内的变异性。通过使用这些方法，应能大大加强对芬苯达唑体外和体内药代动力学的研究。
