用于校准蛋白质印迹法的蛋白质分子量标准免疫血清。
Immune serum to protein molecular weight standards for calibrating Western blots.
作者信息
Carlone G M, Plikaytis B B, Arko R J
出版信息
Anal Biochem. 1986 May 15;155(1):89-91. doi: 10.1016/0003-2697(86)90229-0.
PMID:3717561
Abstract
A method for calibrating proteins by Western blot analysis is described. Rabbit immune serum, specific for seven protein molecular weight standards, and goat anti-rabbit horseradish peroxidase conjugate are used to visualize standards after electrotransfer onto nitrocellulose. This method can be used as a reagent and procedure control.
摘要
描述了一种通过蛋白质免疫印迹分析校准蛋白质的方法。使用针对七种蛋白质分子量标准品的兔免疫血清和山羊抗兔辣根过氧化物酶偶联物，在将标准品电转移到硝酸纤维素膜上后进行可视化。该方法可用作试剂和程序对照。
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