荧光标准物在蛋白质转移和免疫印迹中的应用。免疫反应性物质分子量的准确估计。
Use of fluorescent standards in protein transfer and immunoblotting. Accurate estimation of the molecular weight of immunoreactive species.
作者信息
Law H, Lingwood C A
出版信息
Anal Biochem. 1985 Sep;149(2):404-8. doi: 10.1016/0003-2697(85)90590-1.
PMID:3935006
Abstract
Fluorescent protein molecular weight markers have been used to standardize the electrotransfer and subsequent immunostaining of proteins separated by sodium dodecyl sulfate-polyacrylamide electrophoresis. The method permits accurate estimation of the molecular weight of immunoreactive species despite shrinkage or swelling which may occur during transfer or staining.
摘要
荧光蛋白分子量标记物已被用于标准化通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的蛋白质的电转移及后续免疫染色。该方法能够准确估计免疫反应性物质的分子量，尽管在转移或染色过程中可能会出现收缩或膨胀。
Zotero 插件