Schill W B, Pritsch W, Preissler G
Int J Fertil. 1979;24(1):27-32.
Preservation of human semen in liquid nitrogen causes a significant impairment of sperm motility. Ejaculated human spermatozoa show an increased motility in the presence of caffeine, a phosphodiesterase inhibitor, and pancreatic kallikrein (EC 3.4.21.8), a kinin-producing proteinase. Hence, the effect of both substances on post-thaw motility, fructose consumption, and cervical mucus penetration of cryo-preserved human spermatozoa was investigated. The results indicate that both substances stimulate the motility of freshly ejaculated spermatozoa and also improve the motility pattern of cryo-preserved human spermatozoa, thus offering a possible means of improving the quality of freeze-preserved human semen.
将人类精液保存在液氮中会导致精子活力显著受损。射出的人类精子在磷酸二酯酶抑制剂咖啡因和激肽生成蛋白酶胰激肽释放酶（EC 3.4.21.8）存在时，活力会增强。因此，研究了这两种物质对冷冻保存的人类精子解冻后活力、果糖消耗及穿透宫颈黏液能力的影响。结果表明，这两种物质既能刺激刚射出精子的活力，也能改善冷冻保存的人类精子的活力模式，从而为提高冷冻保存的人类精液质量提供了一种可能的方法。
