Tashiro H, Watanabe K, Takizawa H, Shibazaki Y, Yoshida H
Department of Urology, Showa University School of Medicine.
Nihon Hinyokika Gakkai Zasshi. 1992 Feb;83(2):190-6. doi: 10.5980/jpnjurol1989.83.190.
Cryopreservation of semen from patients with oligozoospermia (sperm counts less than 20 million/ml) generally results in a severe decline in the percentage of motile spermatozoa after thawing in comparison with normozoospermia. We studied the effects of two metabolically active compounds, namely kallikrein and caffeine, on the survival of frozen-stored spermatozoa, in particular, from patients with oligozoospermia. A modified Ackerman's solution was used as the protective medium, and the specimens were frozen using a programmed freezer. We evaluated the post-thaw survival rate of spermatozoa by sperm counts on addition of agents. 1) When used the modified Ackerman's solution only, the post-thaw survival rate in the cases of oligozoospermia was significantly lower than that in the cases of normozoospermia. On the other hand, between these groups, there were no significant differences in the post-thaw activity rates of spermatozoa. 2) When added 1 KE/ml kallikrein and 7.5 mM caffeine (final volume) to the protective medium, the survival rate in the cases of oligozoospermia increased significantly, so that no significant differences were seen in the survival rates between the groups of oligozoospermia and normozoospermia. The effect of caffeine was better than that of kallikrein. 3) It was suggested that kallikrein and caffeine activated the non-motile live spermatozoa after thawing especially in the cases of oligozoospermia.
与正常精子症相比，少精子症（精子计数低于2000万/毫升）患者精液的冷冻保存通常会导致解冻后活动精子百分比严重下降。我们研究了两种代谢活性化合物，即激肽释放酶和咖啡因，对冷冻保存精子，特别是少精子症患者精子存活的影响。使用改良的阿克曼溶液作为保护介质，并使用程序冷冻仪对标本进行冷冻。我们通过添加试剂后的精子计数来评估精子解冻后的存活率。1）仅使用改良的阿克曼溶液时，少精子症患者解冻后的存活率显著低于正常精子症患者。另一方面，在这些组之间，精子解冻后的活动率没有显著差异。2）当在保护介质中添加1KE/毫升激肽释放酶和7.5毫摩尔咖啡因（终体积）时，少精子症患者的存活率显著提高，因此少精子症组和正常精子症组之间的存活率没有显著差异。咖啡因的效果优于激肽释放酶。3）提示激肽释放酶和咖啡因尤其在少精子症患者中能激活解冻后的非活动活精子。
