Department of Biological and Environmental Science and Technology, University of Salento, Via Lecce-Monteroni, 73100 Lecce, Italy.
Department of Mathematics and Physics "E. De Giorgi", University of Salento, Via Lecce-Arnesano, 73100 Lecce, Italy.
Int J Mol Sci. 2023 May 8;24(9):8459. doi: 10.3390/ijms24098459.
Serum samples from eight participants during the XV winter-over at Concordia base (Antarctic expedition) collected at defined time points, including predeparture, constituted the key substrates for a specific metabolomics study. To ascertain acute changes and chronic adaptation to hypoxia, the metabolic profiles of the serum samples were analyzed using NMR spectroscopy, with principal components analysis (PCA) followed by partial least squares and orthogonal partial least squares discriminant analyses (PLS-DA and OPLS-DA) used as supervised classification methods. Multivariate data analyses clearly highlighted an adaptation period characterized by an increase in the levels of circulating glutamine and lipids, mobilized to supply the body energy needs. At the same time, a reduction in the circulating levels of glutamate and -acetyl glycoproteins, stress condition indicators, and proinflammatory markers were also found in the NMR data investigation. Subsequent pathway analysis showed possible perturbations in metabolic processes, potentially related to the physiological adaptation, predominantly found by comparing the baseline (at sea level, before mission onset), the base arrival, and the mission ending collected values.
在康科迪亚基地（南极考察）进行的第 XV 次越冬期间，从 8 名参与者身上采集的血清样本，在特定的时间点进行了采集，这些样本构成了一项特定代谢组学研究的关键底物。为了确定急性变化和对低氧的慢性适应，使用 NMR 光谱法分析了血清样本的代谢谱，使用主成分分析（PCA），随后是偏最小二乘和正交偏最小二乘判别分析（PLS-DA 和 OPLS-DA）作为有监督的分类方法。多变量数据分析清楚地突出了一个适应期，其特征是循环谷氨酰胺和脂质水平增加，以提供身体的能量需求。同时，在 NMR 数据研究中还发现，谷氨酸和 -乙酰糖蛋白（应激条件指标和促炎标志物）的循环水平降低。随后的途径分析显示，代谢过程可能出现了干扰，这可能与生理适应有关，主要是通过比较基线（在海平面，任务开始前）、基地到达和任务结束时采集的值来发现。
