Aly Salah M, Eissa Alaa Eldin, Abdel-Razek Nashwa, El-Ramlawy Asmaa O
Department of Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Department of Aquatic Animal Medicine & Management, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Int J Vet Sci Med. 2023 May 8;11(1):38-54. doi: 10.1080/23144599.2023.2205338. eCollection 2023.
Recently, nanoparticles have attracted attention as a preventive tool for certain infectious diseases affecting fish in aquaculture. Furthermore, freshwater fishes are frequently vulnerable to summer mass morality caused by Aeromonas bacteria. In this regard, we focused on the evaluation of the in vitro and in vivo antimicrobial activity of chitosan (CNPs) and silver (AgNPs) nanoparticles against . CNPs and AgNPs were prepared at a mean particle size of 9.03 and 12.8 nm and a charge equalled+36.4 and -19.3 mV for CNPs and AgNPs, respectively. were retrieved and identified by traditional and molecular techniques. The sensitivity of the obtained bacteria to eight different antibiotic discs was also tested. The antibiotic sensitivity studies revealed the presence of multidrug-resistant (MDR) species (spp.). The bacterium that showed the highest multidrug resistance against the tested antibiotic discs was . Therefore, CNPs and AgNPs were in vitro tested against the isolated bacterium and exhibited inhibition zones of 15 and 25 mm, respectively. TEM images also showed that CNPs and AgNPs had an antagonistic action against the same bacterium causing loss of architecture and bacterial death.
最近，纳米颗粒作为一种预防水产养殖中某些影响鱼类的传染病的工具受到了关注。此外，淡水鱼经常易受气单胞菌引起的夏季大量死亡的影响。在这方面，我们专注于评估壳聚糖（CNPs）和银（AgNPs）纳米颗粒对……的体外和体内抗菌活性。制备的CNPs和AgNPs的平均粒径分别为9.03和12.8纳米，CNPs和AgNPs的电荷分别为+36.4和 -19.3毫伏。通过传统和分子技术检索并鉴定了……。还测试了所获得的细菌对八种不同抗生素圆盘的敏感性。抗生素敏感性研究揭示了多药耐药（MDR）……物种（spp.）的存在。对测试抗生素圆盘显示出最高多药耐药性的细菌是……。因此，对分离出的细菌进行了CNPs和AgNPs的体外测试，它们分别表现出15和25毫米的抑菌圈。透射电子显微镜（TEM）图像还显示，CNPs和AgNPs对同一细菌具有拮抗作用，导致细菌结构丧失和死亡。
