肝素、组胺和鲑鱼降钙素对小鼠颅骨骨吸收的影响。
Effects of heparin, histamine, and salmon calcitonin on mouse calvarial bone resorption.
作者信息
Crisp A J, Wright J K, Hazleman B L
出版信息
Ann Rheum Dis. 1986 May;45(5):422-7. doi: 10.1136/ard.45.5.422.
A quantitative mouse calvarial bone resorption assay was employed to investigate the effects of the mast cell products, heparin and histamine, and of salmon calcitonin. 'Amorphous' heparin, containing a range of molecular weight fractions, inhibited resorption by 15-20% at concentrations of 0.75-5.0 mg/ml. A 'defined' heparin species of mol.wt 13 500 inhibited resorption by 14-28% at 10(-5)-10(-4) mol/l. Histamine inhibited resorption by 19-55% at 10(-3)-10(-2) mol/l. It is proposed that heparin and histamine depress coupled bone resorption and formation and may lead to net loss of bone. Salmon calcitonin inhibited resorption at concentrations as low as 10 pg/ml. 'Amorphous' (but not 'defined') heparin blunted calcitonin induced inhibition of bone resorption and may derepress osteoclasts.
采用定量小鼠颅骨骨吸收试验来研究肥大细胞产物、肝素和组胺以及鲑鱼降钙素的作用。含有一系列分子量级分的“无定形”肝素，在浓度为0.75 - 5.0毫克/毫升时，抑制骨吸收15 - 20%。分子量为13500的“特定”肝素在10⁻⁵ - 10⁻⁴摩尔/升时，抑制骨吸收14 - 28%。组胺在10⁻³ - 10⁻²摩尔/升时，抑制骨吸收19 - 55%。有人提出，肝素和组胺会抑制骨吸收与形成的耦合，可能导致骨的净丢失。鲑鱼降钙素在低至10皮克/毫升的浓度时就能抑制骨吸收。“无定形”（而非“特定”）肝素会减弱降钙素诱导的骨吸收抑制作用，可能会解除对破骨细胞的抑制。
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