Cao Hang, Fang Chao, Wang Qiong, Liu Ling-Ling, Liu Wu-Jun
College of Animal Science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China.
Int J Genomics. 2023 May 4;2023:9934684. doi: 10.1155/2023/9934684. eCollection 2023.
Bovine respiratory disease (BRD) is one of the major health issues in the cattle industry, resulting in significant financial crises globally. There is currently no good treatment, and cattle are made resistant to pneumonia through disease-resistant breeding. The serial blood samples from six Xinjiang brown (XJB) calves were collected for the RNA sequencing (RNA-seq). The obtained six samples were grouped into two groups, in each group as infected with BRD and healthy calves, respectively. In our study, the differential expression mRNAs were detected by using RNA-seq and constructed a protein-protein interaction (PPI) network related to the immunity in cattle. The key genes were identified by protein interaction network analysis, and the results from RNA-seq were verified using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A total of 488 differentially expressed (DE) mRNAs were identified. Importantly, the enrichment analysis of these identified DEGs classified them as mainly enriched in the regulation and immune response processes. The 16 hub genes were found to be related to immune pathways categorized by PPIs analysis. Results revealed that many hub genes were related to the immune response to respiratory disease. These results will provide the basis for a better understanding of the molecular mechanism of bovine resistance to BRD.
牛呼吸道疾病（BRD）是养牛业中的主要健康问题之一，在全球范围内导致重大经济危机。目前尚无有效的治疗方法，通过抗病育种使牛对肺炎产生抗性。采集了6头新疆褐牛（XJB）犊牛的系列血样用于RNA测序（RNA-seq）。将获得的6个样本分为两组，每组分别为感染BRD的犊牛和健康犊牛。在我们的研究中，使用RNA-seq检测差异表达的mRNA，并构建了与牛免疫相关的蛋白质-蛋白质相互作用（PPI）网络。通过蛋白质相互作用网络分析鉴定关键基因，并使用逆转录定量聚合酶链反应（RT-qPCR）验证RNA-seq的结果。共鉴定出488个差异表达（DE）mRNA。重要的是，对这些鉴定出的DEG进行的富集分析将它们分类为主要富集在调节和免疫反应过程中。通过PPI分析发现16个枢纽基因与免疫途径相关。结果表明，许多枢纽基因与对呼吸道疾病的免疫反应有关。这些结果将为更好地理解牛对BRD抗性的分子机制提供依据。
