Roriz Mariana, Pereira Sofia I A, Castro Paula M L, Carvalho Susana M P, Vasconcelos Marta W
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005, Porto, Portugal.
GreenUPorto - Research Centre on Sustainable Agrifood Production / Inov4Agro & DGAOT, Faculty of Sciences, University of Porto, Campus de Vairão, Rua da Agrária 747, 4485-646, Vairão, Portugal.
Heliyon. 2023 Apr 18;9(5):e14620. doi: 10.1016/j.heliyon.2023.e14620. eCollection 2023 May.
Conventional strategies to manage iron (Fe) deficiency still present drawbacks, and more eco-sustainable solutions are needed. Knowledge on soybean-specific diversity and functional traits of their plant growth-promoting bacteria (PGPB) potentiates their applicability as bioinoculants to foster soybean performance under calcareous soil conditions. This work aimed to assess the efficacy of PGPB, retrieved from soybean tissues/rhizosphere, in enhancing plant growth and development as well as crop yield under alkaline soil conditions. Seventy-six bacterial strains were isolated from shoots (18%), roots (53%), and rhizosphere (29%) of soybean. Twenty-nine genera were identified, with and being the most predominant. Based on distinct plant growth-promoting traits, the endophyte P2.3 and the rhizobacteria S2.14 were selected as bioinoculants. tests showed that soybean photosynthetic parameters, chlorophyll content, total fresh weight, and Fe concentrations were not significantly affected by bioinoculation. However, inoculation with P2.3 increased pod number (33%) and the expression of Fe-related genes (FRO2, IRT1, F6'H1, bHLH38, and FER4), and decreased FC-R activity (45%). Moreover, bioinoculation significantly affected Mn, Zn, and Ca accumulation in plant tissues. Soybean harbors several bacterial strains in their tissues and in the rhizosphere with capacities related to Fe nutrition and plant growth promotion. The strain P2.3 showed the best potential to be incorporated in bioinoculant formulations for enhancing soybean performance under alkaline soil conditions.
传统的缺铁管理策略仍然存在缺陷，因此需要更具生态可持续性的解决方案。了解大豆特定的多样性及其植物促生细菌（PGPB）的功能特性，有助于将其作为生物菌剂应用于石灰性土壤条件下，以促进大豆生长。这项工作旨在评估从大豆组织/根际获取的PGPB在碱性土壤条件下促进植物生长发育以及提高作物产量方面的功效。从大豆的地上部分（18%）、根部（53%）和根际（29%）分离出76株细菌菌株。鉴定出29个属，其中[具体属名1]和[具体属名2]最为常见。基于不同的植物促生特性，选择内生菌[菌株名1] P2.3和根际细菌[菌株名2] S2.14作为生物菌剂。[具体测试名称]测试表明，生物菌剂接种对大豆光合参数、叶绿素含量、总鲜重和铁浓度没有显著影响。然而，接种[菌株名1] P2.3可使豆荚数量增加（33%），铁相关基因（FRO2、IRT1、F6'H1、bHLH38和FER4）的表达上调，并降低FC-R活性（45%）。此外，生物菌剂接种显著影响植物组织中锰、锌和钙的积累。大豆在其组织和根际中含有多种细菌菌株，这些菌株具有与铁营养和植物生长促进相关的能力。菌株[菌株名1] P2.3在碱性土壤条件下增强大豆性能方面，显示出最适合用于生物菌剂配方的潜力。
