Ni Shuo, Wu Yamiao, Zhu Ning, Leng Feifan, Wang Yonggang
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
Microorganisms. 2024 Sep 8;12(9):1861. doi: 10.3390/microorganisms12091861.
, commonly known as Dangshen, is a valuable medicinal plant, but its slow growth and susceptibility to environmental stress pose challenges for its cultivation. In pursuit of sustainable agricultural practices to enhance the yield and quality of Dangshen, the present study isolated a bacterial strain exhibiting plant growth-promoting potential from the rhizosphere of . This strain was subsequently identified as YB06. Assessment of its plant growth-promoting attributes revealed the potential of YB06 as a biofertilizer. Whole-genome sequencing of YB06 revealed a genome size of 4,226,888 bp with a GC content of 46.22%, harboring 4325 predicted protein-coding sequences. Genomic analysis of YB06 revealed a diverse array of genes linked to induced systemic resistance (ISR) and plant growth-promoting (PGP) traits, encompassing phytohormone production, nitrogen assimilation and reduction, siderophore biosynthesis, phosphate solubilization, biofilm formation, synthesis of PGP-related amino acids, and flagellar motility. Seed germination assays demonstrated the positive effects of YB06 on the germination and growth of seedlings. Furthermore, we explored various fertilization regimes, particularly the YB06-based biofertilizer, were investigated for their impact on the structure and diversity of the rhizosphere soil bacterial community. Our findings revealed that fertilization significantly impacted soil bacterial composition and diversity, with the combined application of YB06-based biofertilizer and organic fertilizer exhibiting a particularly pronounced enhancement of rhizosphere bacterial community structure and diversity. This study represents the first report on the beneficial effects of YB06 on both the growth of and the composition of its rhizosphere soil microbial community. These findings provide a theoretical foundation and practical guidance for the development of novel bio-organic compound fertilizers, thereby contributing to the sustainable cultivation of .
，俗称党参，是一种珍贵的药用植物，但其生长缓慢且易受环境胁迫影响，给其种植带来了挑战。为了寻求可持续农业实践以提高党参的产量和质量，本研究从 的根际分离出一株具有促进植物生长潜力的细菌菌株。该菌株随后被鉴定为YB06。对其促进植物生长特性的评估揭示了YB06作为生物肥料的潜力。YB06的全基因组测序显示基因组大小为4,226,888 bp，GC含量为46.22%，含有4325个预测的蛋白质编码序列。对YB06的基因组分析揭示了一系列与诱导系统抗性（ISR）和促进植物生长（PGP）特性相关的基因，包括植物激素产生、氮同化和还原、铁载体生物合成、磷溶解、生物膜形成、PGP相关氨基酸的合成以及鞭毛运动。种子发芽试验证明了YB06对党参幼苗发芽和生长的积极影响。此外，我们探索了各种施肥方案，特别是研究了基于YB06的生物肥料对党参根际土壤细菌群落结构和多样性的影响。我们的研究结果表明，施肥显著影响土壤细菌组成和多样性，基于YB06的生物肥料与有机肥料的联合应用对根际细菌群落结构和多样性的增强尤为显著。本研究首次报道了YB06对党参生长及其根际土壤微生物群落组成的有益影响。这些发现为新型生物有机复合肥料的开发提供了理论基础和实践指导，从而有助于党参的可持续种植。
