Møller U, Larsen J K
Cell Tissue Kinet. 1979 Mar;12(2):203-11. doi: 10.1111/j.1365-2184.1979.tb00126.x.
Ultrasonication of keratinized, stratified, squamous epithelium, which had been separated from underlying tissue by means of acetic acid, resulted in disaggregation of all cellular layers in the epithelium, giving a suspension of single nuclei with mitoses preserved. This suspension was treated with RNAse and ethidium bromide for analysis by flow cytometry. From the resulting DNA histogram the G1, S and G2 + M fractions were estimated using the computer program of Fried (1976). Treatment with dithiothreitol before sonication increased the yield of nuclei in suspension and decreased the amount of debris and clumps, thereby suppressing overestimation of small S fractions. This method of preparation prior to DNA flow cytometry was useful for the study of the hamster cheek pouch epithelium and of normal and pathological human epidermis.
对角化的复层鳞状上皮进行超声处理，该上皮已通过乙酸与下层组织分离，结果上皮中的所有细胞层均解聚，得到保存有有丝分裂的单核悬浮液。该悬浮液用RNA酶和溴化乙锭处理，用于流式细胞术分析。根据所得的DNA直方图，使用Fried（1976年）的计算机程序估算G1、S和G2 + M组分。超声处理前用二硫苏糖醇处理可提高悬浮液中细胞核的产量，并减少碎片和团块的数量，从而抑制对小S组分的高估。这种DNA流式细胞术之前的制备方法对于研究仓鼠颊囊上皮以及正常和病理状态下的人类表皮很有用。
