Frentz G, Møller U, Christensen I
J Invest Dermatol. 1980 Mar;74(3):119-21. doi: 10.1111/1523-1747.ep12535006.
A method for single cell DNA measurements by flow cytometry based on a technique successfully used for the hamster cheek pouch epithelium was extended for studies on human epidermis. A least squares estimation procedure was used for the statistical analysis of the initially obtained DNA frequency distribution. In 14 specimens of abdominal skin from a young healthy female the precentages of epidermal cells with an S-phase DNA content were 3.0 +/- 0.6 (SD), and the percentages of epidermal cells with a G2+M phase DNA content 1.1 +/- 0.1 (SD). No significant differences in values were found for specimens investigated in different experiments. No influence of tissue storage time for a maximum of 32 days in liquid nitrogen was discernible. Values from the corresponding dermal tissue were significantly more variable then the epidermal values, but the levels were of the same magnitude. It is concluded that this technique used for human epidermis yields sufficient stable and accurate estimates of S and G2+M fractions that it should be a valuable tool useful as an alternative or a supplement to conventional cell kinetic methods.
一种基于成功应用于仓鼠颊囊上皮细胞的技术，通过流式细胞术进行单细胞DNA测量的方法被扩展用于人体表皮研究。采用最小二乘法估计程序对最初获得的DNA频率分布进行统计分析。在一名年轻健康女性的14份腹部皮肤样本中，处于S期DNA含量的表皮细胞百分比为3.0±0.6（标准差），处于G2+M期DNA含量的表皮细胞百分比为1.1±0.1（标准差）。在不同实验中研究的样本在数值上未发现显著差异。未观察到在液氮中最多保存32天的组织储存时间的影响。相应真皮组织的值比表皮值的变异性明显更大，但水平处于相同数量级。结论是，这种用于人体表皮的技术能够对S期和G2+M期细胞比例产生足够稳定和准确的估计，它应该是一种有价值的工具，可作为传统细胞动力学方法的替代或补充。
