Yu Qingcai, Qian Lisheng, Qiu Wanwei, Miao Yongmei, Zhang Jing, Wang Yan
School of Life and Health Science, Anhui Science and Technology University Fengyang 233100 China
RSC Adv. 2023 May 9;13(20):13940-13946. doi: 10.1039/d3ra00757j. eCollection 2023 May 2.
Human papillomavirus type 16 (HPV16), one of the high-risk types, is responsible for 53% of cervical cancers. The development of an early diagnostic approach with high sensitivity, low-cost, point-of-care testing (POCT) for HPV16 is urgent. In our work, a novel dual-functional AuPt nanoalloy-based lateral flow nucleic acid biosensor (AuPt nanoalloy-based LFNAB) was established with excellent sensitivity for detecting HPV16 DNA for the first time. The AuPt nanoalloy particles were prepared by a one-step reduction method, which was simple, rapid, and green. The AuPt nanoalloy particles retained the performance of initial Au nanoparticles owing to the catalytic activity enabled by Pt. Such dual-functionalities offered two kinds of detection alternatives (, normal mode and amplification mode, respectively). The former is produced just by the black color from the AuPt nanoalloy material itself, and the latter is more color sensitive from its superior catalytic activity. The optimized AuPt nanoalloy-based LFNAB exhibited satisfactory quantitative ability in detecting the target HPV16 DNA in the range of 5-200 pM with a LOD of 0.8 pM at the "amplification mode". The proposed dual-functional AuPt nanoalloy-based LFNAB displayed great potential and promising opportunity in POCT clinical diagnostics.
16型人乳头瘤病毒（HPV16）是高危型之一，引发了53%的宫颈癌。因此，迫切需要开发一种针对HPV16的高灵敏度、低成本、即时检测（POCT）的早期诊断方法。在我们的工作中，首次建立了一种基于新型双功能金铂纳米合金的侧向流动核酸生物传感器（基于金铂纳米合金的LFNAB），对检测HPV16 DNA具有出色的灵敏度。金铂纳米合金颗粒通过一步还原法制备，该方法简单、快速且环保。由于铂赋予的催化活性，金铂纳米合金颗粒保留了初始金纳米颗粒的性能。这种双功能提供了两种检测方式（分别为正常模式和放大模式）。前者仅由金铂纳米合金材料本身产生的黑色显示，后者因其卓越的催化活性而对颜色更敏感。优化后的基于金铂纳米合金的LFNAB在“放大模式”下检测目标HPV16 DNA时，在5-200 pM范围内表现出令人满意的定量能力，检测限为0.8 pM。所提出的基于双功能金铂纳米合金的LFNAB在POCT临床诊断中显示出巨大的潜力和广阔的前景。
