Institute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, 233100, Anhui, China.
School of Biomedical Engineering, Shenzhen University Healthy Science Center, Shenzhen, Guangdong, 518060, People's Republic of China.
Mikrochim Acta. 2021 Mar 21;188(4):133. doi: 10.1007/s00604-021-04788-z.
A gold nanorod (AuNR)-based lateral flow nucleic acid biosensor (LFNAB) is reported for visual detection of DNA with a short test time and high sensitivity. AuNRs with an approximate length of 60 nm were utilized as a colored tag to label the detection DNA probe (Det-DNA). The capture DNA probe (Cap-DNA) was immobilized on the test region of LFNAB. Sandwich-type complex was formed among the AuNR-Det-DNA, target DNA (Tar-DNA), and Cap-DNA on the LFNAB by Watson-Crick base pairing. In the presence of Tar-DNA, AuNRs were thus seized on the test region of LFNAB, and the accumulation of AuNRs subsequently produced a characteristic colored band. The optimized LFNAB was able to detect 10 pM Tar-DNA without instrumentation. Quantitative analysis could be established by measuring the intensity of test band using a portable strip reader, and the detection limit of 2 pM target DNA was achieved on the LFNAB without signal amplification. The detection limit of the AuNR-based LFNAB is 250-fold lower than that of gold nanoparticle (AuNP)-based LFNABs. This work unveiled a sensitive, rapid, and economical strategy for the detection of nucleic acids, and simultaneously opening new promising routes for disease diagnosis and clinical applications. Gold nanorods are used as colored tags for lateral flow nucleic acid biosensor.
一种基于金纳米棒（AuNR）的侧向流核酸生物传感器（LFNAB）被报道用于在短测试时间内实现高灵敏度的 DNA 可视化检测。具有约 60nm 长度的 AuNR 被用作标记检测 DNA 探针（Det-DNA）的彩色标记物。捕获 DNA 探针（Cap-DNA）被固定在 LFNAB 的测试区域上。在 LFNAB 上，通过 Watson-Crick 碱基配对，形成了 AuNR-Det-DNA、靶 DNA（Tar-DNA）和 Cap-DNA 之间的三明治型复合物。在存在 Tar-DNA 的情况下，AuNR 因此被捕获在 LFNAB 的测试区域上，AuNR 的积累随后产生了特征性的有色带。优化后的 LFNAB 无需仪器即可检测到 10pM 的 Tar-DNA。通过使用便携式条带读取器测量测试带的强度，可以建立定量分析，并且在没有信号放大的情况下，在 LFNAB 上可以实现 2pM 目标 DNA 的检测限。基于 AuNR 的 LFNAB 的检测限比基于金纳米粒子（AuNP）的 LFNAB 低 250 倍。这项工作揭示了一种用于核酸检测的灵敏、快速和经济的策略，同时为疾病诊断和临床应用开辟了新的有前途的途径。金纳米棒被用作侧向流核酸生物传感器的彩色标记物。
