PROIMI, CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Av. Belgrano y Pje. Caseros, Tucumán, Argentina.
PROIMI, CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Av. Belgrano y Pje. Caseros, Tucumán, Argentina; Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.
Sci Total Environ. 2023 Aug 20;887:164014. doi: 10.1016/j.scitotenv.2023.164014. Epub 2023 May 12.
Vinasse is a by-product with a key role in circular economy. In this work, we analyze sugarcane vinasse as culture medium for obtaining single and mixed inoculants. Trichoderma harzianum MT2 was cultured in single and sequential co-culture with Pseudomonas capeferrum WCS358 or Rhizobium sp. N21.2. Fungal biomass in single culture was more than three folds higher in vinasse than in a standard medium, and was higher in co-culture with Rhizobium sp. N21.2 than with P. capeferrum WCS358. Bacterial growths in vinasse, in particular P. capeferrum WCS358, were improved in co-culture with T. harzianum MT2. Residual vinasses, obtained after microbial growth, presented almost neutral pH and lower conductivities and toxicity than raw vinasse. Fertigation with residual vinasses modifies characteristics of soil evidenced in the total N, cation exchange capacity, urease and acid phosphatase, and microbial metabolic diversity, in comparison to raw vinasse. In general, soil fertigation with residual vinasse from co-culture with P. capeferrum WCS358 is more similar to irrigation with water. Treatment evaluation indicates that vinasse is suitable for the production of mixed inoculants containing T. harzianum. The co-culture with P. capeferrum WCS358 improves the characteristics of the residual vinasse allowing a fertigation with less detrimental effect in soil in comparison to Rhizobium sp. N21.2. Obtaining valuable biomass of single or mixed inoculants in vinasse with lower ecological impact is relevant for the circular and green economy.
酒糟是循环经济中的一种重要副产物。在这项工作中，我们分析了甘蔗酒糟作为获得单一和混合接种剂的培养基。哈茨木霉 MT2 分别在单一和顺序共培养中与 Capeferrum 假单胞菌 WCS358 或 Rhizobium sp. N21.2 一起培养。在单一培养中，真菌生物量在酒糟中的是标准培养基中的三倍以上，并且与 Rhizobium sp. N21.2 共培养时比与 Capeferrum 假单胞菌 WCS358 共培养时更高。酒糟中细菌的生长，特别是 Capeferrum 假单胞菌 WCS358 的生长，在与哈茨木霉 MT2 共培养时得到了改善。从微生物生长中获得的剩余酒糟的 pH 值接近中性，电导率和毒性比原始酒糟低。与原始酒糟相比，用剩余酒糟进行灌溉会改变土壤的特性，表现在总氮、阳离子交换能力、脲酶和酸性磷酸酶以及微生物代谢多样性方面。一般来说，与原始酒糟相比，用 Capeferrum 假单胞菌 WCS358 的剩余酒糟进行土壤灌溉更类似于用水灌溉。处理评估表明，酒糟适合生产含有哈茨木霉的混合接种剂。与 Rhizobium sp. N21.2 相比，与 Capeferrum 假单胞菌 WCS358 的共培养改善了剩余酒糟的特性，从而可以减少对土壤的不利影响。在酒糟中以较低的生态影响获得有价值的单一或混合接种剂的生物量对于循环绿色经济是相关的。
