Division of Research and Innovations, Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Science, Chennai, 602 105, Tamil Nadu, India.
Department of Chemistry PRIST Deemed to be University Thanjavur, Tamil Nadu, India.
Environ Res. 2023 Aug 15;231(Pt 1):116112. doi: 10.1016/j.envres.2023.116112. Epub 2023 May 13.
This study was designed to appraise the antioxidant and anticancer competence of solvent extracts of Tecoma stans (Linn) and analyze the phytoligands interaction against Bcl 2 VEGFR2 through in silico studies. The phytochemical analysis revealed that the ethyl acetate extract contains more number of pharmaceutically valuable phytochemicals than other solvent extracts. Among the various phytochemicals, flavonoid was found as a predominant component, and UV-Vis- spectrophotometer analysis initially confirmed it. Hence, the column chromatogram was performed to purify the flavonoid, and High-performance liquid chromatography (HPLC) was performed. It revealed that the flavonoid enriched fraction by compared with standard flavonoid molecules. About 84.69% and 80.43% of antioxidant activity were found from ethyl acetate extract of bark and flower at the dosage of 80 μg mL with the IC value of 47.24 and 43.40 μg mL respectively. In a dose-dependent mode, the ethyl acetate extract of bark and flower showed cytotoxicity against breast cancer cell line MCF 7 (Michigan Cancer Foundation-7) as up to 81.38% and 80.94% of cytotoxicity respectively. Furthermore, the IC was found as 208.507 μg mL and 207.38 μg mL for bark and flower extract correspondingly. About 10 medicinal valued flavonoid components were identified from bark (6) and flower (4) ethyl acetate extract through LC-MS analysis. Out of 10 components, the 3,5-O-dicaffeoylquinic acid (ΔG -8.8) and Isorhamnetin-3-O-rutinoside (ΔG -8.3) had the competence to interact with Bcl 2 (B-Cell Lymphoma 2) and VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) respectively with more energy. Hence, these results confirm that the ethyl acetate extract of bark and flower of T. stans has significant medicinal potential and could be used as antioxidant and anticancer agent after some animal performance study.
这项研究旨在评估黄蝉（Tecoma stans（Linn））溶剂提取物的抗氧化和抗癌能力，并通过计算机模拟研究分析植物配体与 Bcl 2 和 VEGFR2 的相互作用。植物化学分析表明，乙酸乙酯提取物比其他溶剂提取物含有更多数量的有药用价值的植物化学物质。在各种植物化学物质中，黄酮类化合物被发现是主要成分，并且紫外可见分光光度计分析初步证实了这一点。因此，进行了柱色谱以纯化黄酮类化合物，并进行了高效液相色谱（HPLC）分析。结果表明，与标准黄酮类分子相比，黄酮类化合物富集部分的含量更高。在 80μg/mL 的剂量下，树皮和花的乙酸乙酯提取物的抗氧化活性分别达到 84.69%和 80.43%，IC 值分别为 47.24μg/mL 和 43.40μg/mL。在剂量依赖性模式下，树皮和花的乙酸乙酯提取物对乳腺癌细胞系 MCF 7（密歇根癌症基金会-7）表现出细胞毒性，细胞毒性分别达到 81.38%和 80.94%。此外，树皮和花提取物的 IC 值分别为 208.507μg/mL 和 207.38μg/mL。通过 LC-MS 分析，从树皮（6）和花（4）的乙酸乙酯提取物中鉴定出约 10 种具有药用价值的黄酮类成分。在这 10 种成分中，3,5-O-二咖啡酰奎宁酸（ΔG-8.8）和异鼠李素-3-O-芦丁苷（ΔG-8.3）分别与 Bcl 2（B 细胞淋巴瘤 2）和 VEGFR2（血管内皮生长因子受体 2）具有相互作用的能力，且能量更高。因此，这些结果证实，T. stans 的树皮和花的乙酸乙酯提取物具有显著的药用潜力，在经过一些动物性能研究后，可以用作抗氧化剂和抗癌剂。
