College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Jiangsu Biodep Biotechnology Co., Ltd., Jiangyin, Jiangsu 214400, PR China; Probiotics Australia Pty, Ormeau, Queensland 4208, Australia.
Carbohydr Polym. 2023 Aug 1;313:120874. doi: 10.1016/j.carbpol.2023.120874. Epub 2023 Apr 1.
The aim of this study is to report the preparation of pectin microspheres by varying degrees of methyl esterification (DM) cross-linked with divalent cationic calcium to encapsulate Lactiplantibacillus plantarum STB1 and L. plantarum LJ1, respectively. Scanning electron microscopy revealed the compact and smooth surface of pectin of DM 28 %, and the stochastic distribution of L. plantarum throughout the gel reticulation. And the pectin of DM 28 % considerably increased probiotics tolerance after continuous exposure to stimulated gastrointestinal tract conditions, with viable counts exceeding 10 CFU/mL. This data indicated that low methoxy-esterification pectin was more efficient to improve the targeted delivery of probiotics in GIT. Additionally, the controlled release of microspheres was dependent on various pH levels. At pH 7.4, the release rates of L. plantarum STB1 and L. plantarum LJ1 reached up to 97.63 % and 95.33 %, respectively. Finally, the Caco-2 cell adhesion model was used to evaluate the cell adhesion rate after encapsulation, which exhibited better adhesion at DM of 60 %.
本研究旨在报道通过不同酯化程度（DM）交联二价阳离子钙来制备果胶微球，分别包埋植物乳杆菌 STB1 和植物乳杆菌 LJ1。扫描电子显微镜显示 DM 28%的果胶表面紧凑光滑，植物乳杆菌随机分布在凝胶网状结构中。并且 DM 28%的果胶显著提高了益生菌在连续暴露于模拟胃肠道条件下的耐受性，活菌数超过 10 CFU/mL。这些数据表明低甲氧基酯化果胶更有效地提高了益生菌在胃肠道中的靶向传递效率。此外，微球的释放速率取决于不同的 pH 值。在 pH 7.4 时，植物乳杆菌 STB1 和植物乳杆菌 LJ1 的释放率分别达到 97.63%和 95.33%。最后，使用 Caco-2 细胞黏附模型评估包封后的细胞黏附率，结果表明在 DM 为 60%时具有更好的黏附性。
