Tianjin Key Laboratory of Pulp and Paper, State Key Laboratory of Biobased Fiber Manufacturing Technology, China Light Industry Key Laboratory of Papermaking and Biorefinery, Tianjin University of Science and Technology, No. 29, 13th Street, TEDA, Tianjin, 300457, PR China.
Tianjin Key Laboratory of Pulp and Paper, State Key Laboratory of Biobased Fiber Manufacturing Technology, China Light Industry Key Laboratory of Papermaking and Biorefinery, Tianjin University of Science and Technology, No. 29, 13th Street, TEDA, Tianjin, 300457, PR China; Limerick Pulp and Paper Centre, University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada.
Carbohydr Polym. 2023 Aug 1;313:120885. doi: 10.1016/j.carbpol.2023.120885. Epub 2023 Apr 5.
Tobacco based cellulose nanofiber (TCNF) is a novel nanocellulose that has recently been used to replace undesirable wood pulp fibers in the preparation of reconstructed tobacco sheets (RTS). However, given the strict requirements for controlling toxic chemical content in tobacco products, there is a global interest in developing a green, efficient, and toxic-chemical free approach to isolate TCNF from tobacco stem as a bioresource. In this study, we propose a creative and environmentally friendly method to efficiently and safely isolate TCNF from tobacco stem pulp, which involves integrated biological pretreatment followed by a facile mechanical defibrillation process. Feruloyl esterase is used to pretreat the stem pulp by disrupting the ether and ester bonds between lignin and polysaccharide carbohydrates within the fiber wall, which effectively facilitates cellulase hydrolysis and swelling of the stem pulp fiber, as well as the following mechanical shearing treatment for TCNF isolation. The results demonstrate that TCNF obtained by the comprehensive feruloyl esterase/cellulase/mechanical process exhibit uniform and well-dispersed nanofiber morphology, higher crystallinity, and stronger mechanical properties than those of the control. The addition of 0.5 % TCNF can replace wood pulp by 18 wt% ~ 25 wt% in the production of RTS samples while maintaining their reasonable strength properties.
以烟草为原料的纤维素纳米纤维（TCNF）是一种新型纳米纤维素，最近已被用于替代再造烟草薄片（RTS）制备中不理想的木浆纤维。然而，鉴于对控制烟草产品中有毒化学物质含量的严格要求，人们对开发一种绿色、高效、无毒化学物质的方法从烟草茎中分离 TCNF 作为生物资源产生了全球性的兴趣。在本研究中，我们提出了一种创新的、环保的方法，可有效地、安全地从烟草茎浆中分离 TCNF，该方法涉及综合生物预处理，然后进行简单的机械纤维细化处理。使用阿魏酸酯酶预处理茎浆，破坏纤维壁中木质素与多糖碳水化合物之间的醚键和酯键，这有效地促进了纤维素酶水解和茎浆纤维的溶胀，以及随后的机械剪切处理以分离 TCNF。结果表明，通过综合的阿魏酸酯酶/纤维素酶/机械处理获得的 TCNF 表现出均匀且分散良好的纳米纤维形态、更高的结晶度和更强的机械性能，优于对照。在 RTS 样品的生产中，添加 0.5%的 TCNF 可以替代 18wt%~25wt%的木浆，同时保持其合理的强度性能。
