State Key Laboratory of Grassland and Agro-ecosystems, School of Life Sciences, Lanzhou University, Lanzhou 730000, PR China; Probiotics and Biological Feed Research Center, Lanzhou University, Lanzhou 730000, PR China.
State Key Laboratory of Grassland and Agro-ecosystems, School of Life Sciences, Lanzhou University, Lanzhou 730000, PR China; Probiotics and Biological Feed Research Center, Lanzhou University, Lanzhou 730000, PR China.
Bioresour Technol. 2020 Aug;310:123476. doi: 10.1016/j.biortech.2020.123476. Epub 2020 May 5.
The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated. Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD). No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp. The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.
研究了产阿魏酸酯酶植物乳杆菌 A1、纤维素酶及其组合对混合玉米秸秆和马铃薯浆青贮发酵特性、碳水化合物组成和酶解的影响。使用了两种混合物比例：复水玉米秸秆与马铃薯浆的重量比为 35:1（HD）和干玉米秸秆与马铃薯浆的重量比为 5:11（LD）。单独添加菌株 A1 对木质纤维素降解和纤维素转化没有优势，而其与纤维素酶的组合增强了木质纤维素降解，并在共青贮玉米秸秆和马铃薯浆中保留了更多可发酵的碳水化合物。酶解结果表明预处理对沼气生产有潜在的好处，因为未经添加剂处理的共青贮 HD 比例混合物在发酵 60 天后进行酶解，葡萄糖产量很高。
