Lopes Kittelson Katiana Simões, Faustino Martins Allana Cristina, Celante Gizele, Gasparotto Arquimedes, da Silva Gomes Roberto
Laboratory of Cardiovascular Pharmacology (LaFaC), Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS, Brazil.
Department of Pharmaceutical Sciences, College of Health and Human Sciences, North Dakota State University (NDSU), Fargo, ND, United States.
Int J Innov Sci Res Technol. 2025 Mar;10(3):3307-3319. doi: 10.38124/ijisrt/25mar736. Epub 2025 Apr 29.
Considering the number of available methods in molecular and cellular biology and the rapid development of new technologies, the need for an updated guide on what is currently applied and how to choose the best method for a specific goal has increased. Gold standard methods are known for their accuracy and reliable data under certain circumstances, allowing replicability. This perspective aims to characterize and define the current gold standard techniques from the last five years, enlighten their historical background, cite drawbacks and benefits, and delimit possible future aspects.
鉴于分子和细胞生物学中现有方法的数量以及新技术的快速发展,对于一份关于当前应用了什么以及如何为特定目标选择最佳方法的最新指南的需求与日俱增。金标准方法以其在特定情况下的准确性和可靠数据而闻名,具备可重复性。本观点旨在对过去五年的当前金标准技术进行特征描述和定义,阐明其历史背景,列举缺点和优点,并界定可能的未来发展方向。