人尿中抗肿瘤组分的研究。该组分中主要蛋白质的特性分析。
Studies on an antineoplastic fraction from human urine. Characterization of the major protein in this fraction.
作者信息
Sloane N H, Lynn W R, Macleod R M, Hade E P, Pottathil R, Kyriazis A P
出版信息
Biochem J. 1986 Mar 1;234(2):355-62. doi: 10.1042/bj2340355.
A fraction has been isolated from human urine which exhibits antiproliferative activity against human tumour cell lines without affecting the growth of several normal diploid cell lines or tumour cells of mouse or hamster origin. The major protein present in this fraction has been characterized and tentatively designated antineoplastic urinary protein (ANUP). An S020,W value of 3.69 S was obtained by sedimentation velocity analysis, and a subunit molecular mass of 16 300 Da was obtained by sedimentation equilibrium and by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Centrifugation data also indicated that the protein self-associates. The amino acid analysis of ANUP was consistent with its low pI (4.2) as determined by chromatofocusing analysis. Furthermore, the amino acid composition exhibited some features similar to collagen, as shown by high levels of proline and glycine, the absence of cysteine, and the presence of low levels of hydroxyproline.
从人尿中分离出一种成分，它对人肿瘤细胞系具有抗增殖活性，却不影响几种正常二倍体细胞系或源自小鼠或仓鼠的肿瘤细胞的生长。该成分中的主要蛋白质已被鉴定，并暂定为抗肿瘤尿蛋白（ANUP）。通过沉降速度分析得到的S020,W值为3.69 S，通过沉降平衡和十二烷基硫酸钠/聚丙烯酰胺凝胶电泳得到的亚基分子量为16300 Da。离心数据还表明该蛋白质会自我缔合。ANUP的氨基酸分析结果与其通过色谱聚焦分析测定的低等电点（4.2）一致。此外，氨基酸组成表现出一些与胶原蛋白相似的特征，如脯氨酸和甘氨酸含量高、半胱氨酸缺失以及羟脯氨酸含量低。
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